| RRC ID |
70442
|
| Author |
Haney MG, Wimsett M, Liu C, Blackburn JS.
|
| Title |
Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models.
|
| Journal |
STAR Protoc
|
| Abstract |
Dysregulation of Wnt signaling is a hallmark of many cancers, and the development of effective, non-toxic small-molecule Wnt inhibitors is desirable. Off-target toxicities of new compounds are typically tested in mouse models, which is both costly and time consuming. Here, we present a rapid and inexpensive protocol to determine the in vivo toxicity and efficacy of novel Wnt inhibitors in zebrafish using a combination of a fluorescence reporter assay as well as eye rescue and fin regeneration assays. These experiments are completed within 1 week to rapidly narrow drug candidates before moving to more expensive pre-clinical testing. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).
|
| Volume |
2(2)
|
| Pages |
100433
|
| Published |
2021-6-18
|
| DOI |
10.1016/j.xpro.2021.100433
|
| PII |
S2666-1667(21)00140-4
|
| PMID |
33889852
|
| PMC |
PMC8050796
|
| MeSH |
Animals
Antineoplastic Agents* / pharmacology
Antineoplastic Agents* / toxicity
Drug Evaluation, Preclinical / methods*
Female
Male
Neoplasms, Experimental / metabolism
Wnt Proteins / antagonists & inhibitors*
Wnt Proteins / metabolism
Wnt Signaling Pathway / drug effects*
Zebrafish
|
| Resource |
| Zebrafish |
Tg(Tcf/Lef-miniP:dGFP) |
