RRC ID 70743
Author Gebert D, Neubert LK, Lloyd C, Gui J, Lehmann R, Teixeira FK.
Title Large Drosophila germline piRNA clusters are evolutionarily labile and dispensable for transposon regulation.
Journal Mol Cell
Abstract PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility and transposon defense in the animal germline. In most species, the majority of piRNAs are produced from distinct large genomic loci, called piRNA clusters. It is assumed that germline-expressed piRNA clusters, particularly in Drosophila, act as principal regulators to control transposons dispersed across the genome. Here, using synteny analysis, we show that large clusters are evolutionarily labile, arise at loci characterized by recurrent chromosomal rearrangements, and are mostly species-specific across the Drosophila genus. By engineering chromosomal deletions in D. melanogaster, we demonstrate that the three largest germline clusters, which account for the accumulation of >40% of all transposon-targeting piRNAs in ovaries, are neither required for fertility nor for transposon regulation in trans. We provide further evidence that dispersed elements, rather than the regulatory action of large Drosophila germline clusters in trans, may be central for transposon defense.
Volume 81(19)
Pages 3965-3978.e5
Published 2021-10-7
DOI 10.1016/j.molcel.2021.07.011
PII S1097-2765(21)00580-3
PMID 34352205
PMC PMC8516431
MeSH Animals Animals, Genetically Modified Argonaute Proteins / genetics Argonaute Proteins / metabolism Chromosome Deletion Chromosomes, Insect DNA Transposable Elements* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Evolution, Molecular* Female Fertility / genetics* Gene Expression Regulation, Developmental Multigene Family* Ovary / metabolism Ovary / physiology* RNA Stability* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism
IF 15.584
Drosophila DGRC#124205 DGRC#126282