論文 - 詳細
| RRC ID | 70743 |
|---|---|
| 著者 | Gebert D, Neubert LK, Lloyd C, Gui J, Lehmann R, Teixeira FK. |
| タイトル | Large Drosophila germline piRNA clusters are evolutionarily labile and dispensable for transposon regulation. |
| ジャーナル | Mol Cell |
| Abstract |
PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility and transposon defense in the animal germline. In most species, the majority of piRNAs are produced from distinct large genomic loci, called piRNA clusters. It is assumed that germline-expressed piRNA clusters, particularly in Drosophila, act as principal regulators to control transposons dispersed across the genome. Here, using synteny analysis, we show that large clusters are evolutionarily labile, arise at loci characterized by recurrent chromosomal rearrangements, and are mostly species-specific across the Drosophila genus. By engineering chromosomal deletions in D. melanogaster, we demonstrate that the three largest germline clusters, which account for the accumulation of >40% of all transposon-targeting piRNAs in ovaries, are neither required for fertility nor for transposon regulation in trans. We provide further evidence that dispersed elements, rather than the regulatory action of large Drosophila germline clusters in trans, may be central for transposon defense. |
| 巻・号 | 81(19) |
| ページ | 3965-3978.e5 |
| 公開日 | 2021-10-7 |
| DOI | 10.1016/j.molcel.2021.07.011 |
| PII | S1097-2765(21)00580-3 |
| PMID | 34352205 |
| PMC | PMC8516431 |
| MeSH | Animals Animals, Genetically Modified Argonaute Proteins / genetics Argonaute Proteins / metabolism Chromosome Deletion Chromosomes, Insect DNA Transposable Elements* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Evolution, Molecular* Female Fertility / genetics* Gene Expression Regulation, Developmental Multigene Family* Ovary / metabolism Ovary / physiology* RNA Stability* RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism |
| IF | 15.584 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 77 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#124205 DGRC#126282 |