RRC ID 70745
Author Moussian B, Casadei N.
Title Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes.
Journal Methods Mol Biol
Abstract RNA processing is a vital process in all organisms. In eukaryotes, the RNA induced silencing complex (RISC) mediates this function during development and physiological processes and, at least in arthropods, during RNA-viral infections. Argonaute-like RNA-binding proteins are central components of this complex. RNA-based insecticides are gaining more and more a central role in pest control. Understanding of the underlying molecular mechanisms including Ago-like proteins is crucial in designing powerful, species-specific and environmental-friendly insecticides. This chapter describes a protocol for identification and genetic functional analyses of insect Ago-like proteins in the fruit fly Drosophila melanogaster that serves as a living test tube.
Volume 2360
Pages 9-17
Published 2022-1-1
DOI 10.1007/978-1-0716-1633-8_2
PMID 34495503
MeSH Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Drosophila / metabolism Drosophila Proteins / genetics Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Genome, Insect* Insecticides RNA RNA Interference RNA-Induced Silencing Complex / metabolism