論文 - 詳細
| RRC ID | 73431 |
|---|---|
| 著者 | Averbeck NB, Barent C, Jakob B, Syzonenko T, Durante M, Taucher-Scholz G. |
| タイトル | The Ubiquitin Ligase RNF138 Cooperates with CtIP to Stimulate Resection of Complex DNA Double-Strand Breaks in Human G1-Phase Cells. |
| ジャーナル | Cells |
| Abstract |
DNA double-strand breaks (DSBs) represent the molecular origin of ionizing-radiation inflicted biological effects. An increase in the ionization density causes more complex, clustered DSBs that can be processed by resection also in G1 phase, where repair of resected DSBs is considered erroneous and may contribute to the increased biological effectiveness of heavy ions in radiotherapy. To investigate the resection regulation of complex DSBs, we exposed G1 cells depleted for different candidate factors to heavy ions or α-particle radiation. Immunofluorescence microscopy was used to monitor the resection marker RPA, the DSB marker γH2AX and the cell-cycle markers CENP-F and geminin. The Fucci system allowed to select G1 cells, cell survival was measured by clonogenic assay. We show that in G1 phase the ubiquitin ligase RNF138 functions in resection regulation. RNF138 ubiquitinates the resection factor CtIP in a radiation-dependent manner to allow its DSB recruitment in G1 cells. At complex DSBs, RNF138's participation becomes more relevant, consistent with the observation that also resection is more frequent at these DSBs. Furthermore, deficiency of RNF138 affects both DSB repair and cell survival upon induction of complex DSBs. We conclude that RNF138 is a regulator of resection that is influenced by DSB complexity and can affect the quality of DSB repair in G1 cells. |
| 巻・号 | 11(16) |
| 公開日 | 2022-8-17 |
| DOI | 10.3390/cells11162561 |
| PII | cells11162561 |
| PMID | 36010636 |
| PMC | PMC9406464 |
| MeSH | Carrier Proteins / genetics DNA DNA Breaks, Double-Stranded* Endodeoxyribonucleases / metabolism* G1 Phase / genetics Humans Ligases Ubiquitin* / genetics Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism |
| IF | 4.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |