| RRC ID |
73461
|
| Author |
Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K.
|
| Title |
Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination.
|
| Journal |
Exp Anim
|
| Abstract |
The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression in vivo. In most Cre-reporter mouse strains, although the presence of reporter product indicates the expression of Cre recombinase, it has remained unclear whether a lack of reporter signal indicates either no Cre recombinase expression or insufficient reporter gene promoter activity. We produced a novel ROSA26 knock-in Cre-reporter C57BL/6N strain exhibiting green emission before and red after Cre-mediated recombination, designated as strain R26GRR. Ubiquitous green fluorescence and no red fluorescence were observed in R26GRR mice. To investigate the activation of tdsRed, EGFP-excised R26GRR, R26RR, mice were produced through the crossing of C57BL/6N mice with R26GRR/Ayu1-Cre F1 mice. R26RR mice showed extraordinarily strong red fluorescence in almost all tissues examined, suggesting ubiquitous activation of the second reporter in all tissues after Cre/loxP recombination. Moreover, endothelial cell lineage and pancreatic islet-specific expression of red fluorescence were detected in R26GRR/Tie2-Cre F1 mice and R26GRR /Ins1-Cre F1 mice, respectively. These results indicated that R26GRR mice are a useful novel Cre-reporter mouse strain. In addition, R26GRR mice with a pure C57BL/6N background represent a valuable source of green-to-red photoconvertible cells following Cre/loxP recombination for application in transplantation studies. The R26GRR mouse strain will be available from RIKEN BioResource Center (http://www.brc.riken.jp/lab/animal/en/).
|
| Volume |
62(4)
|
| Pages |
295-304
|
| Published |
2013-1-1
|
| DOI |
10.1538/expanim.62.295
|
| PII |
DN/JST.JSTAGE/expanim/62.295
|
| PMID |
24172193
|
| PMC |
PMC4160954
|
| MeSH |
Animals
Cells, Cultured
Embryonic Stem Cells
Endothelial Cells / metabolism
Female
Gene Expression*
Gene Knock-In Techniques
Genes, Reporter / genetics*
Genes, Reporter / physiology*
Green Fluorescent Proteins / metabolism*
Integrases / genetics*
Integrases / metabolism*
Islets of Langerhans / metabolism
Luminescent Proteins
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Recombination, Genetic
Ubiquitination
|
| IF |
1.574
|
| Resource |
| DNA material |
pROSA26CAG-GFP-tdsRed (RDB19441) |
