論文 - 詳細
| RRC ID | 73461 |
|---|---|
| 著者 | Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K. |
| タイトル | Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. |
| ジャーナル | Exp Anim |
| Abstract |
The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression in vivo. In most Cre-reporter mouse strains, although the presence of reporter product indicates the expression of Cre recombinase, it has remained unclear whether a lack of reporter signal indicates either no Cre recombinase expression or insufficient reporter gene promoter activity. We produced a novel ROSA26 knock-in Cre-reporter C57BL/6N strain exhibiting green emission before and red after Cre-mediated recombination, designated as strain R26GRR. Ubiquitous green fluorescence and no red fluorescence were observed in R26GRR mice. To investigate the activation of tdsRed, EGFP-excised R26GRR, R26RR, mice were produced through the crossing of C57BL/6N mice with R26GRR/Ayu1-Cre F1 mice. R26RR mice showed extraordinarily strong red fluorescence in almost all tissues examined, suggesting ubiquitous activation of the second reporter in all tissues after Cre/loxP recombination. Moreover, endothelial cell lineage and pancreatic islet-specific expression of red fluorescence were detected in R26GRR/Tie2-Cre F1 mice and R26GRR /Ins1-Cre F1 mice, respectively. These results indicated that R26GRR mice are a useful novel Cre-reporter mouse strain. In addition, R26GRR mice with a pure C57BL/6N background represent a valuable source of green-to-red photoconvertible cells following Cre/loxP recombination for application in transplantation studies. The R26GRR mouse strain will be available from RIKEN BioResource Center (http://www.brc.riken.jp/lab/animal/en/). |
| 巻・号 | 62(4) |
| ページ | 295-304 |
| 公開日 | 2013-1-1 |
| DOI | 10.1538/expanim.62.295 |
| PII | DN/JST.JSTAGE/expanim/62.295 |
| PMID | 24172193 |
| PMC | PMC4160954 |
| MeSH | Animals Cells, Cultured Embryonic Stem Cells Endothelial Cells / metabolism Female Gene Expression* Gene Knock-In Techniques Genes, Reporter / genetics* Genes, Reporter / physiology* Green Fluorescent Proteins / metabolism* Integrases / genetics* Integrases / metabolism* Islets of Langerhans / metabolism Luminescent Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Recombination, Genetic Ubiquitination |
| IF | 1.574 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pROSA26CAG-GFP-tdsRed (RDB19441) |