RRC ID 73746
Author Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T.
Title An ultramarine fluorescent protein with increased photostability and pH insensitivity.
Journal Nat Methods
Abstract We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells.
Volume 6(5)
Pages 351-3
Published 2009-5-1
DOI 10.1038/nmeth.1317
PII nmeth.1317
PMID 19349978
MeSH Amino Acid Substitution Animals Apoptosis Calcium Signaling Caspase 3 / metabolism Color Dictyostelium / microbiology Escherichia coli / genetics Escherichia coli / metabolism Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / genetics HeLa Cells Humans Hydrogen-Ion Concentration Luminescent Proteins / chemistry* Luminescent Proteins / genetics Molecular Sequence Data Mutagenesis Phagocytosis Photobleaching Protein Stability Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Transfection
IF 30.822
DNA material Sirius/pcDNA3 (RDB19875)