Reference - Detail
RRC ID | 73746 |
---|---|
Author | Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T. |
Title | An ultramarine fluorescent protein with increased photostability and pH insensitivity. |
Journal | Nat Methods |
Abstract |
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells. |
Volume | 6(5) |
Pages | 351-3 |
Published | 2009-5-1 |
DOI | 10.1038/nmeth.1317 |
PII | nmeth.1317 |
PMID | 19349978 |
MeSH | Amino Acid Substitution Animals Apoptosis Calcium Signaling Caspase 3 / metabolism Color Dictyostelium / microbiology Escherichia coli / genetics Escherichia coli / metabolism Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / genetics HeLa Cells Humans Hydrogen-Ion Concentration Luminescent Proteins / chemistry* Luminescent Proteins / genetics Molecular Sequence Data Mutagenesis Phagocytosis Photobleaching Protein Stability Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Transfection |
IF | 30.822 |
Resource | |
DNA material | Sirius/pcDNA3 (RDB19875) |