RRC ID 74946
Author Krüger LK, Gélin M, Ji L, Kikuti C, Houdusse A, Théry M, Blanchoin L, Tran PT.
Title Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.
Journal Elife
Abstract Mitotic spindle function depends on the precise regulation of microtubule dynamics and microtubule sliding. Throughout mitosis, both processes have to be orchestrated to establish and maintain spindle stability. We show that during anaphase B spindle elongation in Schizosaccharomyces pombe, the sliding motor Klp9 (kinesin-6) also promotes microtubule growth in vivo. In vitro, Klp9 can enhance and dampen microtubule growth, depending on the tubulin concentration. This indicates that the motor is able to promote and block tubulin subunit incorporation into the microtubule lattice in order to set a well-defined microtubule growth velocity. Moreover, Klp9 recruitment to spindle microtubules is dependent on its dephosphorylation mediated by XMAP215/Dis1, a microtubule polymerase, creating a link between the regulation of spindle length and spindle elongation velocity. Collectively, we unravel the mechanism of anaphase B, from Klp9 recruitment to the motors dual-function in regulating microtubule sliding and microtubule growth, allowing an inherent coordination of both processes.
Volume 10
Published 2021-6-3
DOI 10.7554/eLife.67489
PII 67489
PMID 34080538
PMC PMC8205488
MeSH Gene Expression Regulation, Fungal Kinesins / genetics Kinesins / metabolism* Meiosis* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Microtubules / genetics Microtubules / metabolism* Molecular Motor Proteins / genetics Molecular Motor Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction Spindle Apparatus / genetics Spindle Apparatus / metabolism* Time Factors
IF 7.08