RRC ID 75746
Author Mizoguchi T, Togawa S, Kawakami K, Itoh M.
Title Neuron and sensory epithelial cell fate is sequentially determined by Notch signaling in zebrafish lateral line development.
Journal J Neurosci
Abstract Sensory systems are specialized to recognize environmental changes. Sensory organs are complex structures composed of different cell types, including neurons and sensory receptor cells, and how these organs are generated is an important question in developmental neurobiology. The posterior lateral line (pLL) is a simple sensory system in fish and amphibians that detects changes in water motion. It consists of neurons and sensory receptor hair cells, both of which are derived from the cranial ectoderm preplacodal region. However, it is not clearly understood how neurons and the sensory epithelium develop separately from the same preplacodal progenitors. We found that the numbers of posterior lateral line ganglion (pLLG) neurons, which are marked by neurod expression, increased in embryos with reduced Notch activity, but the forced activation of Notch reduced their number, suggesting that Notch-mediated lateral inhibition regulates the pLLG cell fate in zebrafish. By fate-mapping analysis, we found that cells adjacent to the pLLG neurons in the pre-pLL placodal region gave rise to the anterior part of the pLL primordium (i.e., sensory epithelial progenitor cells), and that the choice of cell fate between pLLG neuron or pLL primordium was regulated by Notch signaling. Since Notch signaling also affects hair cell fate determination at a later stage, our study suggests that Notch signaling has dual, time-dependent roles in specifying multiple cell types during pLL development.
Volume 31(43)
Pages 15522-30
Published 2011-10-26
DOI 10.1523/JNEUROSCI.3948-11.2011
PII 31/43/15522
PMID 22031898
PMC PMC6703510
MeSH Animals Animals, Genetically Modified Embryo, Nonmammalian Epithelial Cells / drug effects Epithelial Cells / metabolism* Gene Expression Regulation, Developmental / drug effects Gene Expression Regulation, Developmental / genetics Green Fluorescent Proteins / genetics Homeodomain Proteins / metabolism Lateral Line System / cytology* Lateral Line System / embryology* Models, Biological Nerve Tissue Proteins / metabolism Neurons / drug effects Neurons / metabolism* Oligodeoxyribonucleotides, Antisense / pharmacology Receptors, Notch / classification Receptors, Notch / genetics Receptors, Notch / metabolism* Signal Transduction / drug effects Signal Transduction / genetics Signal Transduction / physiology* Time Factors Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.674
Zebrafish hspGFFDMC72A