論文 - 詳細
| RRC ID | 76028 |
|---|---|
| 著者 | Pastore B, Hertz HL, Price IF, Tang W. |
| タイトル | pre-piRNA trimming and 2'-O-methylation protect piRNAs from 3' tailing and degradation in C. elegans. |
| ジャーナル | Cell Rep |
| Abstract |
The Piwi-interacting RNA (piRNA) pathway suppresses transposable elements and promotes fertility in diverse organisms. Maturation of piRNAs involves pre-piRNA trimming followed by 2'-O-methylation at their 3' termini. Here, we report that the 3' termini of Caenorhabditis elegans piRNAs are subject to nontemplated nucleotide addition, and piRNAs with 3' addition exhibit extensive base-pairing interaction with their target RNAs. Animals deficient for PARN-1 (pre-piRNA trimmer) and HENN-1 (2'-O-methyltransferase) accumulate piRNAs with 3' nontemplated nucleotides. In henn-1 mutants, piRNAs are shortened prior to 3' addition, whereas long isoforms of untrimmed piRNAs are preferentially modified in parn-1 mutant animals. Loss of either PARN-1 or HENN-1 results in modest reduction in steady-state levels of piRNAs. Deletion of both enzymes leads to depletion of piRNAs, desilenced piRNA targets, and impaired fecundity. Together, our findings suggest that pre-piRNA trimming and 2'-O-methylation act collaboratively to protect piRNAs from tailing and degradation. |
| 巻・号 | 36(9) |
| ページ | 109640 |
| 公開日 | 2021-8-31 |
| DOI | 10.1016/j.celrep.2021.109640 |
| PII | S2211-1247(21)01083-4 |
| PMID | 34469728 |
| PMC | PMC8459939 |
| MeSH | Animals Animals, Genetically Modified Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Exoribonucleases / genetics Exoribonucleases / metabolism Fertility Methylation Methyltransferases / genetics Methyltransferases / metabolism RNA Precursors / genetics RNA Precursors / metabolism* RNA Processing, Post-Transcriptional* RNA Stability* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4477 tm869 |