| RRC ID |
76067
|
| Author |
Suehiro Y, Yoshina S, Motohashi T, Iwata S, Dejima K, Mitani S.
|
| Title |
Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals.
|
| Journal |
Sci Rep
|
| Abstract |
With the development of massive parallel sequencing technology, it has become easier to establish new model organisms that are ideally suited to the specific biological phenomena of interest. Considering the history of research using classical model organisms, we believe that the efficient construction and sharing of gene mutation libraries will facilitate the progress of studies using these new model organisms. Using C. elegans, we applied the TMP/UV mutagenesis method to animals lacking function in the DNA damage response genes atm-1 and xpc-1. This method produces genetic mutations three times more efficiently than mutagenesis of wild-type animals. Furthermore, we confirmed that the use of next-generation sequencing and the elimination of false positives through machine learning could automate the process of mutation identification with an accuracy of over 95%. Eventually, we sequenced the whole genomes of 488 strains and isolated 981 novel mutations generated by the present method; these strains have been made available to anyone who wants to use them. Since the targeted DNA damage response genes are well conserved and the mutagens used in this study are also effective in a variety of species, we believe that our method is generally applicable to a wide range of animal species.
|
| Volume |
11(1)
|
| Pages |
7630
|
| Published |
2021-4-7
|
| DOI |
10.1038/s41598-021-87226-7
|
| PII |
10.1038/s41598-021-87226-7
|
| PMID |
33828169
|
| PMC |
PMC8027614
|
| MeSH |
Animals
Ataxia Telangiectasia Mutated Proteins / genetics
Base Sequence / genetics
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
DNA / genetics
DNA Damage / genetics
DNA Repair / genetics*
Gene Library
Genetic Techniques
High-Throughput Nucleotide Sequencing / methods
Mutagenesis / genetics
Mutagenesis, Site-Directed / methods*
Mutagens
Mutation / genetics
Phenotype
Sequence Analysis, DNA / methods
|
| Resource |
| C.elegans |
tm764
tm3724
tm1203
tm750
tm1524
tm2026
tm3157
tm5027
tm3886 |
