| RRC ID |
76103
|
| Author |
Zaghet N, Madsen K, Rossi F, Perez DF, Amendola PG, Demharter S, Pfisterer U, Khodosevich K, Pasini D, Salcini AE.
|
| Title |
Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality.
|
| Journal |
Cell Rep
|
| Abstract |
Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.
|
| Volume |
37(8)
|
| Pages |
110050
|
| Published |
2021-11-23
|
| DOI |
10.1016/j.celrep.2021.110050
|
| PII |
S2211-1247(21)01536-9
|
| PMID |
34818537
|
| PMC |
PMC8640224
|
| MeSH |
Animals
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / metabolism
Chromatin / metabolism
Germ Cells / metabolism*
Histone Demethylases / metabolism*
Histone-Lysine N-Methyltransferase / metabolism
Histones / metabolism
Jumonji Domain-Containing Histone Demethylases / metabolism
Methylation
|
| Resource |
| C.elegans |
tm3118
tm3735
tm2828
tm2966
tm3121
tm3197
tm1200 |
