論文 - 詳細
| RRC ID | 77369 |
|---|---|
| 著者 | Man Z, Kondo Y, Koga H, Umino H, Nakayama K, Shin HW. |
| タイトル | Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. |
| ジャーナル | J Biol Chem |
| Abstract |
Arfaptins (arfaptin-1 and arfaptin-2/POR1) were originally identified as binding partners of the Arf small GTPases. Both proteins contain a BAR (Bin/Amphiphysin/Rvs) domain, which participates in membrane deformation. Here we show that arfaptins associate with trans-Golgi membranes. Unexpectedly, Arl1 (Arf-like 1), but not Arfs, determines the trans-Golgi association of arfaptins. We also demonstrate that arfaptins interact with Arl1 through their BAR domain-containing region and compete for Arl1 binding with golgin-97 and golgin-245/p230, both of which also bind to Arl1 through their GRIP (golgin-97/RanBP2/Imh1p/p230) domains. However, arfaptins and these golgins show only limited colocalization at the trans-Golgi. Time-lapse imaging of cells overexpressing fluorescent protein-tagged arfaptins and golgin-97 reveals that arfaptins, but not golgin-97, are included in vesicular and tubular structures emanating from the Golgi region. These observations indicate that arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains. |
| 巻・号 | 286(13) |
| ページ | 11569-78 |
| 公開日 | 2011-4-1 |
| DOI | 10.1074/jbc.M110.201442 |
| PII | S0021-9258(20)53778-2 |
| PMID | 21239483 |
| PMC | PMC3064211 |
| MeSH | ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism* Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Autoantigens / genetics Autoantigens / metabolism Golgi Apparatus / genetics Golgi Apparatus / metabolism* Golgi Matrix Proteins HeLa Cells Humans Intracellular Membranes / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Protein Structure, Tertiary / physiology |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pcDNA3-HA-hArfaptin1 (RDB20097) pcDNA3-HA-hArfaptin2 (RDB20098) EGFP-hArfaptin1 (RDB20099) EGFP-hArfaptin2 (RDB20100) hARL1(WT)-EGFP (RDB20101) hARL1(Q71L)-EGFP (RDB20102) hARL1(T31N)-EGFP (RDB20103) |