RRC ID 82799
Author Ellis K, Bagwell J, Bagnat M.
Title Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis.
Journal J Cell Biol
Abstract The notochord plays critical structural and signaling roles during vertebrate development. At the center of the vertebrate notochord is a large fluid-filled organelle, the notochord vacuole. Although these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenance. Here we show that zebrafish notochord vacuoles are specialized lysosome-related organelles whose formation and maintenance requires late endosomal trafficking regulated by the vacuole-specific Rab32a and H(+)-ATPase-dependent acidification. We establish that notochord vacuoles are required for body axis elongation during embryonic development and identify a novel role in spine morphogenesis. Thus, the vertebrate notochord plays important structural roles beyond early development.
Volume 200(5)
Pages 667-79
Published 2013-3-4
DOI 10.1083/jcb.201212095
PII jcb.201212095
PMID 23460678
PMC PMC3587825
MeSH Animals Animals, Genetically Modified Axis, Cervical Vertebra / embryology Axis, Cervical Vertebra / metabolism Axis, Cervical Vertebra / physiology* Cell Movement Endocytosis Endosomes / metabolism Gene Expression Regulation, Developmental HEK293 Cells Humans Hydrogen-Ion Concentration Lysosomes / metabolism Lysosomes / physiology* Microscopy, Confocal Morphogenesis Notochord / metabolism Notochord / physiology* Protein Transport Proton-Translocating ATPases Recombinant Fusion Proteins / metabolism Spine / embryology Spine / metabolism Spine / physiology* Time Factors Time-Lapse Imaging Transfection Zebrafish / embryology Zebrafish / genetics Zebrafish / metabolism Zebrafish / physiology* Zebrafish Proteins / metabolism rab GTP-Binding Proteins / metabolism
IF 8.811
Resource
Zebrafish SAGFF(LF)214A