RRC ID 867
Author Nakamura T, Kashiwazaki J, Shimoda C.
Title A fission yeast SNAP-25 homologue, SpSec9, is essential for cytokinesis and sporulation.
Journal Cell Struct. Funct.
Abstract The soluble NSF attachment protein 25 (SNAP-25) is a component of the SNARE complex that is essential for regulated exocytosis in diverse cell types. Here, we identified a fission yeast SNAP-25 homologue, SpSec9. The sec9+ gene was essential for vegetative growth. sec9 mRNA was detected in vegetative cells and further increased during sporulation. This increase during sporulation was dependent on Mei4, a meiosis-specific transcription factor. A sporulation-deficient sec9 mutant was isolated by random PCR mutagenesis (sec9-10). The sec9-10 mutant also exhibited temperature sensitivity for growth and cell division was found to arrest before completion of cell separation at restrictive temperatures. In sec9-10 cells, the forespore membrane was normally initiated near spindle pole bodies during meiosis II. However, subsequent extension of the membrane was severely impaired. These results indicate that SpSec9 plays an important role both in cytokinesis and in sporulation.
Volume 30(2)
Pages 15-24
Published 2005
PII JST.JSTAGE/csf/30.15
PMID 16272747
MeSH Amino Acid Sequence Cytokinesis / genetics Cytokinesis / physiology* Gene Expression Regulation, Fungal Gene Targeting Intracellular Membranes / ultrastructure Microscopy, Fluorescence Molecular Sequence Data Mutagenesis Point Mutation Qa-SNARE Proteins / metabolism R-SNARE Proteins / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / physiology* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Sequence Alignment Sequence Homology, Amino Acid Spores, Fungal Synaptosomal-Associated Protein 25 / genetics Synaptosomal-Associated Protein 25 / physiology* Temperature Transcription, Genetic
IF 1.9
Times Cited 24
WOS Category CELL BIOLOGY
Resource
Yeast FY7251(TN4) FY12081(TN52) MKW5 FY6768(MM59-4D) FY12692(JZ670) FY7476(AB4)