Reference - Detail
| RRC ID | 87388 |
|---|---|
| Author | Yu T, Liu X, Tan X, Zhang Y, He Z, Yang W, Tian T, Li Y, Zhao J, Liu C. |
| Title | Identification of renal stem cells in zebrafish. |
| Journal | Sci Adv |
| Abstract |
Renal stem cells (RSC) hold great promise as kidney disease regenerative therapies. However, RSCs capable of regenerating de novo nephrons remain unidentified in vertebrates. Therefore, this study aimed to identify RSCs in zebrafish. Single-cell RNA sequencing revealed eya2, pax2a, and six2a as primary markers of zebrafish RSCs. Real-time imaging demonstrated that RSCs originated from eya2-positive mesenchymal cells. Notably, photoconversion-based lineage tracing and serial transplantation assays revealed a unique RSC renewal process, characterized by a differentiation-proliferation-dedifferentiation mode. This process generates nephrons and nascent RSCs concurrently. In addition, precise Wnt signaling is key for RSC renewal and differentiation balance and directly activates eya2 expression to initiate renewal. This discovery establishes a foundation for the advancement of stem cell therapies for kidney diseases. |
| Volume | 11(34) |
| Pages | eadx5296 |
| Published | 2025-8-22 |
| DOI | 10.1126/sciadv.adx5296 |
| PMID | 40845090 |
| PMC | PMC12372851 |
| MeSH | Animals Biomarkers / metabolism Cell Differentiation Cell Proliferation Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Kidney* / cytology Kidney* / metabolism Nephrons / cytology Nephrons / metabolism PAX2 Transcription Factor / genetics PAX2 Transcription Factor / metabolism Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism Single-Cell Analysis Stem Cells* / cytology Stem Cells* / metabolism Wnt Signaling Pathway Zebrafish* / genetics Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 13.117 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Zebrafish | UAS:GFP |