RRC ID 11352
Author Natsume T, Tsutsui Y, Sutani T, Dunleavy EM, Pidoux AL, Iwasaki H, Shirahige K, Allshire RC, Yamao F.
Title A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.
Journal PLoS ONE
Abstract Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains: the central CENP-A(Cnp1) kinetochore domain and flanking heterochromatin domains. Here we show that fission yeast Mcl1, a DNA polymerase alpha (Pol alpha) accessory protein, is critical for maintenance of centromeric chromatin. In a screen for mutants that alleviate both central domain and outer repeat silencing, we isolated several cos mutants, of which cos1 is allelic to mcl1. The mcl1-101 mutation causes reduced CENP-A(Cnp1) in the central domain and an aberrant increase in histone acetylation in both domains. These phenotypes are also observed in a mutant of swi7(+), which encodes a catalytic subunit of Pol alpha. Mcl1 forms S-phase-specific nuclear foci, which colocalize with those of PCNA and Pol alpha. These results suggest that Mcl1 and Pol alpha are required for propagation of centromere chromatin structures during DNA replication.
Volume 3(5)
Pages e2221
Published 2008-5-21
DOI 10.1371/journal.pone.0002221
PMID 18493607
PMC PMC2376062
MeSH Acetylation Centromere* Chromatin Immunoprecipitation Chromosomes, Fungal Cloning, Molecular DNA Polymerase I / metabolism* Gene Silencing Histone Deacetylases / genetics Histones / metabolism Microscopy, Fluorescence Mutation Myeloid Cell Leukemia Sequence 1 Protein Proto-Oncogene Proteins c-bcl-2 / physiology* Schizosaccharomyces / enzymology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure*
IF 2.776
Times Cited 8
Yeast FY8452