RRC ID 11371
Author Wu JQ, Ye Y, Wang N, Pollard TD, Pringle JR.
Title Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.
Journal Genetics
Abstract A major question about cytokinesis concerns the role of the septin proteins, which localize to the division site in all animal and fungal cells but are essential for cytokinesis only in some cell types. For example, in Schizosaccharomyces pombe, four septins localize to the division site, but deletion of the four genes produces only a modest delay in cell separation. To ask if the S. pombe septins function redundantly in cytokinesis, we conducted a synthetic-lethal screen in a septin-deficient strain and identified seven mutations. One mutation affects Cdc4, a myosin light chain that is an essential component of the cytokinetic actomyosin ring. Five others cause frequent cell lysis during cell separation and map to two loci. These mutations and their dosage suppressors define a signaling pathway (including Rho1 and a novel arrestin) for repairing cell-wall damage. The seventh mutation affects the poorly understood RNA-binding protein Scw1 and severely delays cell separation when combined either with a septin mutation or with a mutation affecting the septin-interacting, anillin-like protein Mid2, suggesting that Scw1 functions in a pathway parallel to that of the septins. Taken together, our results suggest that the S. pombe septins participate redundantly in one or more pathways that cooperate with the actomyosin ring during cytokinesis and that a septin defect causes septum defects that can be repaired effectively only when the cell-integrity pathway is intact.
Volume 186(3)
Pages 897-915
Published 2010-11
DOI 10.1534/genetics.110.119842
PII genetics.110.119842
PMID 20739711
PMC PMC2975288
MeSH Actomyosin / genetics Actomyosin / metabolism* Alleles Cell Division* Genes, Fungal / genetics Genetic Linkage Guanine Nucleotide Exchange Factors / metabolism Models, Biological Mutation / genetics Phenotype Plasmids / genetics Protein Binding Protein Transport Rho Guanine Nucleotide Exchange Factors Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Septins / genetics Septins / metabolism* Signal Transduction*
IF 3.564
Times Cited 23
Yeast FY11065 Plasmid library