論文 - 詳細
RRC ID | 11381 |
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著者 | Zhang Z, Kulkarni K, Hanrahan SJ, Thompson AJ, Barford D. |
タイトル | The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27. |
ジャーナル | EMBO J |
Abstract |
The anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase responsible for controlling cell cycle transitions, is a multisubunit complex assembled from 13 different proteins. Numerous APC/C subunits incorporate multiple copies of the tetratricopeptide repeat (TPR). Here, we report the crystal structure of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units. A C-terminal block of TPR motifs interacts with Hcn1, whereas an N-terminal TPR block mediates Cdc16/Cut9 self-association through a homotypic interface. This dimer interface is structurally related to the N-terminal dimerization domain of Cdc27, demonstrating that both Cdc16/Cut9 and Cdc27 form homo-dimers through a conserved mechanism. The acetylated N-terminal Met residue of Hcn1 is enclosed within a chamber created from the Cut9 TPR superhelix. Thus, in complex with Cdc16/Cut9, the N-acetyl-Met residue of Hcn1, a putative degron for the Doa10 E3 ubiquitin ligase, is inaccessible for Doa10 recognition, protecting Hcn1/Cdc26 from ubiquitin-dependent degradation. This finding may provide a structural explanation for a mechanism to control the stoichiometry of proteins participating in multisubunit complexes. |
巻・号 | 29(21) |
ページ | 3733-44 |
公開日 | 2010-11-3 |
DOI | 10.1038/emboj.2010.247 |
PII | emboj2010247 |
PMID | 20924356 |
PMC | PMC2982764 |
MeSH | Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome Crystallization Crystallography, X-Ray DNA Polymerase III / chemistry* DNA Polymerase III / genetics DNA Polymerase III / metabolism Evolution, Molecular Molecular Sequence Data Nuclear Proteins / chemistry* Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Conformation Protein Multimerization Repetitive Sequences, Amino Acid* Repressor Proteins / chemistry* Repressor Proteins / genetics Repressor Proteins / metabolism Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
IF | 9.889 |
引用数 | 54 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
酵母 | cDNA library |