| RRC ID |
11760
|
| Author |
Ohata S, Aoki R, Kinoshita S, Yamaguchi M, Tsuruoka-Kinoshita S, Tanaka H, Wada H, Watabe S, Tsuboi T, Masai I, Okamoto H.
|
| Title |
Dual roles of Notch in regulation of apically restricted mitosis and apicobasal polarity of neuroepithelial cells.
|
| Journal |
Neuron
|
| Abstract |
How the mitosis of neuroepithelial stem cells is restricted to the apical ventricular area remains unclear. In zebrafish, the mosaic eyes(rw306) (moe/epb41l5(rw306)) mutation disrupts the interaction between the putative adaptor protein Moe and the apicobasal polarity regulator Crumbs (Crb), and impairs the maintenance of neuroepithelial apicobasal polarity. While Crb interacts directly with Notch and inhibits its activity, Moe reverses this inhibition. In the moe(rw306) hindbrain, Notch activity is significantly reduced, and the number of cells that proliferate basally away from the apical area is increased. Surprisingly, activation of Notch in the moe(rw306) mutant rescues not only the basally localized proliferation but also the aberrant neuroepithelial apicobasal polarity. We present evidence that the Crb⋅Moe complex and Notch play key roles in a positive feedback loop to maintain the apicobasal polarity and the apical-high basal-low gradient of Notch activity in neuroepithelial cells, both of which are essential for their apically restricted mitosis.
|
| Volume |
69(2)
|
| Pages |
215-30
|
| Published |
2011-1-27
|
| DOI |
10.1016/j.neuron.2010.12.026
|
| PII |
S0896-6273(10)01074-3
|
| PMID |
21262462
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Movement / physiology
Cell Polarity / physiology*
Embryonic Development / physiology
Eye Proteins / genetics
Eye Proteins / metabolism*
Glycogen Synthase Kinase 3 / metabolism
Glycogen Synthase Kinase 3 beta
Mitosis / physiology*
Motor Neurons / cytology
Motor Neurons / physiology
Mutation
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Neuroepithelial Cells* / cytology
Neuroepithelial Cells* / physiology
Proto-Oncogene Proteins c-akt / metabolism
Receptors, Notch / genetics
Receptors, Notch / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Stem Cells / cytology
Stem Cells / physiology
Zebrafish / anatomy & histology
Zebrafish / physiology
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
| IF |
14.415
|
| Times Cited |
61
|
|
WOS Category
|
NEUROSCIENCES
|
| Resource |
| Zebrafish |
rw306 (mosaic eyes)
rw0 (Tg(CM-isl1:GFP))
Tg(vsx1:EGFP)? |
