Reference - Detail
RRC ID | 12313 |
---|---|
Author | Matsui H, Taniguchi Y, Inoue H, Uemura K, Takeda S, Takahashi R. |
Title | A chemical neurotoxin, MPTP induces Parkinson's disease like phenotype, movement disorders and persistent loss of dopamine neurons in medaka fish. |
Journal | Neurosci Res |
Abstract |
Parkinson's disease (PD) is the second most common neurodegenerative disease associated with the degeneration of dopaminergic neurons in the substantia nigra. To create a new model of PD, we used medaka (Oryzias latipes), a small teleost that has been used in genetics and environmental biology. We identified tyrosine hydroxylase (TH) immunopositive dopaminergic and noradrenergic fibers and neurons in the medaka brain. Following establishment of a method for counting the number of dopaminergic neurons and an assay for the evaluation of the medaka behavior, we exposed medaka to 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine (MPTP). The treatment of medaka at the larval stage, but not at adult stage, decreased the number of dopaminergic cells in the diencephalon and reduced spontaneous movement, which is reminiscent of human PD patients and other MPTP-induced animal PD models. Among TH(+) neurons in the medaka brain, only a specific cluster in the paraventricular area of the middle diencephalon was vulnerable to MPTP toxicity. Detailed examinations of medaka transiently exposed to MPTP at the larval stage revealed that the number of dopaminergic cells was not fully recovered at their adult stage. Moreover, the amounts of dopamine persistently decreased in the brain of these MPTP-treated fish. MPTP-treated medaka is valuable for modeling human PD. |
Volume | 65(3) |
Pages | 263-71 |
Published | 2009-11-1 |
DOI | 10.1016/j.neures.2009.07.010 |
PII | S0168-0102(09)00236-3 |
PMID | 19665499 |
MeSH | Age Factors Animals Biomarkers / metabolism Brain / drug effects* Brain / pathology Brain / physiopathology* Diencephalon / drug effects Diencephalon / pathology Diencephalon / physiopathology Disease Models, Animal* Dopamine / metabolism Dyskinesia, Drug-Induced / metabolism Dyskinesia, Drug-Induced / pathology Dyskinesia, Drug-Induced / physiopathology Larva / drug effects Larva / growth & development Nerve Degeneration / chemically induced Nerve Degeneration / metabolism Nerve Degeneration / physiopathology Neurons / drug effects* Neurons / metabolism Neurons / pathology Oryzias / physiology* Parkinsonian Disorders / metabolism Parkinsonian Disorders / pathology Parkinsonian Disorders / physiopathology* Substantia Nigra / drug effects Substantia Nigra / pathology Substantia Nigra / physiopathology Time |
IF | 2.645 |
Times Cited | 29 |
WOS Category | NEUROSCIENCES |
Resource | |
Medaka | Kyoto-cab (OK-Cab (MT830)) |