RRC ID 12429
著者 Fujimoto A, Akifusa S, Kamio N, Hirofuji T, Nonaka K, Yamashita Y.
タイトル Involvement of mTOR in globular adiponectin-induced generation of reactive oxygen species.
ジャーナル Free Radic Res
Abstract Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. This study investigated the role of the mammalian target of rapamycin (mTOR) in gAd-induced ROS and NO generation. gAd stimulation induced phosphorylation of mTOR, which peaked at 20 min and dissolved rapidly. Inhibition of phosphatidylinositol 3-kinase activity with wortmannin suppressed gAd-induced phosphorylation of Akt and mTOR. Administration of rapamycin partially reduced gAd-induced generation of intracellular and mitochondrial ROS, but not release of NO. To further confirm the role of mTOR in gAd stimulation, the effect of the activators of AMP-activated protein kinase (AMPK) on gAd-induced mTOR phosphorylation was examined. Pre-treatment with three kinds of AMPK activators, AICAR, 2-deoxy-D-glucose and A-769662, suppressed gAd-induced mTOR phosphorylation. Furthermore, these AMPK activators significantly reduced gAd-evoked intracellular and mitochondrial ROS generation and NO release.
巻・号 44(2)
ページ 128-34
公開日 2010-2-1
DOI 10.3109/10715760903348328
PII 10.3109/10715760903348328
PMID 19886750
MeSH Adiponectin / antagonists & inhibitors Adiponectin / pharmacology* Aminoimidazole Carboxamide / analogs & derivatives Aminoimidazole Carboxamide / pharmacology Androstadienes / pharmacology Animals Biphenyl Compounds Cells, Cultured Deoxyglucose / pharmacology Intracellular Signaling Peptides and Proteins / metabolism* Macrophages / drug effects Macrophages / metabolism Mice Nitric Oxide / metabolism Phosphatidylinositol 3-Kinases / metabolism Phosphoinositide-3 Kinase Inhibitors Phosphorylation / drug effects Protein Serine-Threonine Kinases / metabolism* Pyrones / pharmacology Reactive Oxygen Species / metabolism* Ribonucleotides / pharmacology Sirolimus / pharmacology Structure-Activity Relationship TOR Serine-Threonine Kinases Thiophenes / pharmacology Wortmannin
IF 2.839
引用数 10
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 THP-1(RCB1189)