論文 - 詳細
| RRC ID | 12466 |
|---|---|
| 著者 | Yamagishi Y, Honda T, Tanno Y, Watanabe Y. |
| タイトル | Two histone marks establish the inner centromere and chromosome bi-orientation. |
| ジャーナル | Science |
| Abstract |
For proper partitioning of chromosomes in mitosis, the chromosomal passenger complex (CPC) including Aurora B and survivin must be localized at the center of paired kinetochores, at the site called the inner centromere. It is largely unknown what defines the inner centromere and how the CPC is targeted to this site. Here, we show that the phosphorylation of histone H3-threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 (H2A-S121) phosphorylation in targeting the CPC to the inner centromere in fission yeast and human cells. H3-pT3 promotes nucleosome binding of survivin, whereas phosphorylated H2A-S121 facilitates the binding of shugoshin, the centromeric CPC adaptor. Haspin colocalizes with cohesin by associating with Pds5, whereas Bub1 localizes at kinetochores. Thus, the inner centromere is defined by intersection of two histone kinases. |
| 巻・号 | 330(6001) |
| ページ | 239-43 |
| 公開日 | 2010-10-8 |
| DOI | 10.1126/science.1194498 |
| PII | 330/6001/239 |
| PMID | 20929775 |
| MeSH | Amino Acid Sequence Aurora Kinase B Aurora Kinases Cell Cycle Proteins / metabolism Centromere / metabolism* Chromatin / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosome Segregation Chromosomes, Fungal / physiology* Chromosomes, Human / physiology* HeLa Cells Heterochromatin / metabolism Histones / metabolism* Humans Inhibitor of Apoptosis Proteins Intracellular Signaling Peptides and Proteins / chemistry Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Kinetochores / metabolism Microtubule-Associated Proteins / metabolism Mitosis Molecular Sequence Data Mutation Nucleosomes / metabolism Phosphorylation Protein Binding Protein Interaction Domains and Motifs Protein Serine-Threonine Kinases / chemistry Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Serine / metabolism Survivin Threonine / metabolism |
| IF | 41.846 |
| 引用数 | 280 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 酵母 | KM311? |