RRC ID 12524
Author Purtill FS, Whitehall SK, Williams ES, McInerny CJ, Sharrocks AD, Morgan BA.
Title A homeodomain transcription factor regulates the DNA replication checkpoint in yeast.
Journal Cell Cycle
Abstract Checkpoints monitor the successful completion of cell cycle processes, such as DNA replication, and also regulate the expression of cell cycle-dependent genes that are required for responses. In the model yeast Schizosaccharomyces pombe G 1/S phase-specific gene expression is regulated by the MBF (also known as DSC1) transcription factor complex and is also activated by the mammalian ATM/ATR-related Rad3 DNA replication checkpoint. Here, we show that the Yox1 homeodomain transcription factor acts to co-ordinate the expression of MBF-regulated genes during the cell division cycle. Moreover, our data suggests that Yox1 is inactivated by the Rad3 DNA replication checkpoint via phosphorylation by the conserved Cds1 checkpoint kinase. Collectively, our data has implications for understanding the mechanisms underlying the coordination of cell cycle processes in eukaryotes.
Volume 10(4)
Pages 664-70
Published 2011-2-15
DOI 10.4161/cc.10.4.14824
PII 14824
PMID 21304269
PMC PMC3174001
MeSH Ataxia Telangiectasia Mutated Proteins Cell Cycle* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Checkpoint Kinase 2 DNA Replication* DNA, Fungal / genetics DNA, Fungal / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation, Fungal* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Phosphorylation Protein Kinases / genetics Protein Kinases / metabolism Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism
IF 3.259
Times Cited 7
Yeast FY7448