RRC ID 12549
著者 Kühl I, Dujeancourt L, Gaisne M, Herbert CJ, Bonnefoy N.
タイトル A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression.
ジャーナル Nucleic Acids Res
Abstract Pentatricopeptide repeat (PPR) proteins are particularly numerous in plant mitochondria and chloroplasts, where they are involved in different steps of RNA metabolism, probably due to the repeated 35 amino acid PPR motifs that are thought to mediate interactions with RNA. In non-photosynthetic eukaryotes only a handful of PPR proteins exist, for example the human LRPPRC, which is involved in a mitochondrial disease. We have conducted a systematic study of the PPR proteins in the fission yeast Schizosaccharomyces pombe and identified, in addition to the mitochondrial RNA polymerase, eight proteins all of which localized to the mitochondria, and showed some association with the membrane. The absence of all but one of these PPR proteins leads to a respiratory deficiency and modified patterns of steady state mt-mRNAs or newly synthesized mitochondrial proteins. Some cause a general defect, whereas others affect specific mitochondrial RNAs, either coding or non-coding: cox1, cox2, cox3, 15S rRNA, atp9 or atp6, sometimes leading to secondary defects. Interestingly, the two possible homologs of LRPPRC, ppr4 and ppr5, play opposite roles in the expression of the cox1 mt-mRNA, ppr4 being the first mRNA-specific translational activator identified in S. pombe, whereas ppr5 appears to be a general negative regulator of mitochondrial translation.
巻・号 39(18)
ページ 8029-41
公開日 2011-10-1
DOI 10.1093/nar/gkr511
PII gkr511
PMID 21727087
PMC PMC3185421
MeSH Amino Acid Motifs Gene Expression Regulation, Fungal* Genes, Mitochondrial* Genome, Fungal Mitochondria / genetics* Mitochondria / metabolism Mitochondrial Proteins / chemistry Mitochondrial Proteins / genetics Mitochondrial Proteins / physiology* Mutation Phenotype Protein Biosynthesis RNA / metabolism RNA Stability RNA, Mitochondrial Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / physiology* Sequence Homology, Amino Acid
IF 11.502
引用数 32
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pDUAL-FFH1 (RDB06185) S. pombe FLAG2-His
酵母 FY14145 FY14191