RRC ID 12654
著者 Ge W, Chew TG, Wachtler V, Naqvi SN, Balasubramanian MK.
タイトル The novel fission yeast protein Pal1p interacts with Hip1-related Sla2p/End4p and is involved in cellular morphogenesis.
ジャーナル Mol Biol Cell
Abstract The establishment and maintenance of characteristic cellular morphologies is a fundamental property of all cells. Here we describe Schizosaccharomyces pombe Pal1p, a protein important for maintenance of cylindrical cellular morphology. Pal1p is a novel membrane-associated protein that localizes to the growing tips of interphase cells and to the division site in cells undergoing cytokinesis in an F-actin- and microtubule-independent manner. Cells deleted for pal1 display morphological defects, characterized by the occurrence of spherical and pear-shaped cells with an abnormal cell wall. Pal1p physically interacts and displays overlapping localization with the Huntingtin-interacting-protein (Hip1)-related protein Sla2p/End4p, which is also required for establishment of cylindrical cellular morphology. Sla2p is important for efficient localization of Pal1p to the sites of polarized growth and appears to function upstream of Pal1p. Interestingly, spherical pal1Delta mutants polarize to establish a pearlike morphology before mitosis in a manner dependent on the kelch-repeat protein Tea1p and the cell cycle inhibitory kinase Wee1p. Thus, overlapping mechanisms involving Pal1p, Tea1p, and Sla2p contribute to the establishment of cylindrical cellular morphology, which is important for proper spatial regulation of cytokinesis.
巻・号 16(9)
ページ 4124-38
公開日 2005-9-1
DOI 10.1091/mbc.e04-11-0976
PII E04-11-0976
PMID 15975911
PMC PMC1196324
MeSH Actins / metabolism Amino Acid Sequence Cell Wall / metabolism Cytokinesis / physiology Deoxyribonuclease IV (Phage T4-Induced) / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Membrane Proteins / physiology Microtubules / metabolism Mitosis / physiology Molecular Sequence Data Mutation Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Schizosaccharomyces pombe Proteins / physiology
IF 3.791
引用数 28
WOS 分野 CELL BIOLOGY
リソース情報
酵母