| RRC ID |
12711
|
| Author |
Shinada K, Tsukiyama T, Sho T, Okumura F, Asaka M, Hatakeyama S.
|
| Title |
RNF43 interacts with NEDL1 and regulates p53-mediated transcription.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The ubiquitin-proteasomal system plays a crucial role in oncogenesis in colorectal tissues. Recent studies have shown that stability of β-catenin, which functions as an oncogene for colorectal cancer, is regulated by ubiquitin-mediated degradation. It has been reported that a putative E3 ubiquitin ligase, RNF43, is highly expressed in human colorectal carcinoma and that RNF43 promotes cell growth. However, the involvement of RNF43 in carcinogenesis has not been fully elucidated. In this study, we found by using yeast two-hybrid screening that RNF43 binds to NEDD-4-like ubiquitin-protein ligase-1 (NEDL1), which enhances pro-apoptotic activity by p53. In addition, we found that RNF43 also interacts with p53 and that RNF43 suppresses transcriptional activity of p53 in H1299 cells and attenuates apoptosis induced by ultraviolet irradiation. These findings suggest that RNF43 is associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis.
|
| Volume |
404(1)
|
| Pages |
143-7
|
| Published |
2011-1-7
|
| DOI |
10.1016/j.bbrc.2010.11.082
|
| PII |
S0006-291X(10)02148-0
|
| PMID |
21108931
|
| MeSH |
Apoptosis
Carcinoma / genetics
Carcinoma / metabolism*
Carcinoma / pathology
Cell Line, Tumor
Cell Transformation, Neoplastic / genetics
Cell Transformation, Neoplastic / metabolism*
Cell Transformation, Neoplastic / pathology
Colorectal Neoplasms / genetics
Colorectal Neoplasms / metabolism*
Colorectal Neoplasms / pathology
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Gene Expression Regulation
Gene Expression Regulation, Neoplastic
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Oncogene Proteins / genetics
Oncogene Proteins / metabolism*
Transcription, Genetic
Tumor Suppressor Protein p53 / metabolism*
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
Ultraviolet Rays
|
| IF |
2.985
|
| Times Cited |
36
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pp53-Box RE-TK hRluc(F) (RDB05593) |
