RRC ID 12711
Author Shinada K, Tsukiyama T, Sho T, Okumura F, Asaka M, Hatakeyama S.
Title RNF43 interacts with NEDL1 and regulates p53-mediated transcription.
Journal Biochem. Biophys. Res. Commun.
Abstract The ubiquitin-proteasomal system plays a crucial role in oncogenesis in colorectal tissues. Recent studies have shown that stability of β-catenin, which functions as an oncogene for colorectal cancer, is regulated by ubiquitin-mediated degradation. It has been reported that a putative E3 ubiquitin ligase, RNF43, is highly expressed in human colorectal carcinoma and that RNF43 promotes cell growth. However, the involvement of RNF43 in carcinogenesis has not been fully elucidated. In this study, we found by using yeast two-hybrid screening that RNF43 binds to NEDD-4-like ubiquitin-protein ligase-1 (NEDL1), which enhances pro-apoptotic activity by p53. In addition, we found that RNF43 also interacts with p53 and that RNF43 suppresses transcriptional activity of p53 in H1299 cells and attenuates apoptosis induced by ultraviolet irradiation. These findings suggest that RNF43 is associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis.
Volume 404(1)
Pages 143-7
Published 2011-1-7
DOI 10.1016/j.bbrc.2010.11.082
PII S0006-291X(10)02148-0
PMID 21108931
MeSH Apoptosis Carcinoma / genetics Carcinoma / metabolism* Carcinoma / pathology Cell Line, Tumor Cell Transformation, Neoplastic / genetics Cell Transformation, Neoplastic / metabolism* Cell Transformation, Neoplastic / pathology Colorectal Neoplasms / genetics Colorectal Neoplasms / metabolism* Colorectal Neoplasms / pathology DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation Gene Expression Regulation, Neoplastic Humans Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Oncogene Proteins / genetics Oncogene Proteins / metabolism* Transcription, Genetic Tumor Suppressor Protein p53 / metabolism* Two-Hybrid System Techniques Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* Ultraviolet Rays
IF 2.705
Times Cited 19
DNA material pp53-Box RE-TK hRluc(F) (RDB05593)