論文 - 詳細
| RRC ID | 1379 |
|---|---|
| 著者 | Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T. |
| タイトル | Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Hsp105alpha is a 105-kDa stress protein, which is expressed constitutively at especially high levels in the brain compared with other tissues in mammals, and is also induced by a variety of stressors. Recently, we have shown that Hsp105alpha binds to alpha-tubulin and prevents the heat-induced disaggregation of microtubules. To further elucidate the function of Hsp105alpha, we searched for Hsp105alpha-binding proteins by screening a mouse FM3A cell library and human and mouse brain cDNA libraries using the yeast and bacterial two-hybrid systems. We showed here that Hsp105alpha interacted with several cellular proteins, such as cofilin, dynein light chain 2A, alpha-adducin, ubiquitin activating enzyme E1, phosphoglycerate kinase 1, and platelet-activating factor acethylhydrolase alpha1-subunit. The interaction was validated by the results of a pull-down assay and indirect immunofluorescence analysis. The significance of Hsp105alpha and Hsp105alpha-binding proteins in cells was discussed. |
| 巻・号 | 314(2) |
| ページ | 396-402 |
| 公開日 | 2004-2-6 |
| DOI | 10.1016/j.bbrc.2003.12.108 |
| PII | S0006291X03026962 |
| PMID | 14733918 |
| MeSH | Animals Bacterial Proteins / chemistry Brain / metabolism COS Cells Calmodulin-Binding Proteins / metabolism Cytoskeleton / metabolism DNA, Complementary / metabolism Fluorescent Antibody Technique, Indirect Fungal Proteins / chemistry Gene Library HSP110 Heat-Shock Proteins HSP70 Heat-Shock Proteins / metabolism* Humans Mice Microscopy, Fluorescence Microtubules / metabolism Plasmids / metabolism Protein Binding Protein Biosynthesis Recombinant Proteins / metabolism Transcription, Genetic Tubulin / metabolism Two-Hybrid System Techniques* |
| IF | 2.985 |
| 引用数 | 4 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |