論文 - 詳細
| RRC ID | 18118 |
|---|---|
| 著者 | Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M. |
| タイトル | Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation. |
| ジャーナル | J Biol Chem |
| Abstract |
The double-stranded RNA analog, poly(I:C), extracellularly activates both the endosomal Toll-like receptor (TLR) 3 and the cytoplasmic RNA helicase, melanoma differentiation-associated gene 5, leading to the production of type I interferons (IFNs) and inflammatory cytokines. The mechanism by which extracellular poly(I:C) is delivered to TLR3-positive organelles and the cytoplasm remains to be elucidated. Here, we show that the cytoplasmic lipid raft protein, Raftlin, is essential for poly(I:C) cellular uptake in human myeloid dendritic cells and epithelial cells. When Raftlin was silenced, poly(I:C) failed to enter cells and induction of IFN-β production was inhibited. In addition, cellular uptake of B-type oligodeoxynucleotide that shares its uptake receptor with poly(I:C) was suppressed in Raftlin knockdown cells. Upon poly(I:C) stimulation, Raftlin was translocated from the cytoplasm to the plasma membrane where it colocalized with poly(I:C), and thereafter moved to TLR3-positive endosomes. Thus, Raftlin cooperates with the uptake receptor to mediate cell entry of poly(I:C), which is critical for activation of TLR3. |
| 巻・号 | 286(12) |
| ページ | 10702-11 |
| 公開日 | 2011-3-25 |
| DOI | 10.1074/jbc.M110.185793 |
| PII | S0021-9258(20)53893-3 |
| PMID | 21266579 |
| PMC | PMC3060521 |
| MeSH | Animals Cell Line, Tumor Cell Membrane / genetics Cell Membrane / metabolism Dendritic Cells / cytology Dendritic Cells / metabolism* Endosomes / genetics Endosomes / metabolism Gene Silencing HEK293 Cells Humans Interferon Inducers / pharmacology* Interferon-beta / biosynthesis Interferon-beta / genetics Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Knockout Myeloid Cells / cytology Myeloid Cells / metabolism* Poly I-C / pharmacology* Toll-Like Receptor 3 / agonists Toll-Like Receptor 3 / genetics Toll-Like Receptor 3 / metabolism* |
| IF | 4.238 |
| 引用数 | 48 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Namalwa(RCB0445) RAJI(RCB1647) BALL-1(RCB0256) |