RRC ID 19331
著者 Mogi M, Kondo A.
タイトル Down-regulation of mTOR leads to up-regulation of osteoprotegerin in bone marrow cells.
ジャーナル Biochem Biophys Res Commun
Abstract Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor regulates bone mass by inhibiting osteoclastic bone resorption. mTOR, which is the mammalian target of rapamycin, is a kinase and central regulator of cell growth, proliferation, and survival. By using Rapamycin, we studied whether mTOR pathway is associated with OPG protein production in the mouse bone marrow-derived stromal cell line ST2. Rapamycin markedly increased the level of soluble OPG in ST2 cells. This antibiotic treatment resulted in the suppression of phosphorylation of mTOR. Rapamycin had no effects on the proliferation, differentiation, or apoptosis of the cells. Treatment with bone morphogenetic protein-4, which can induce OPG protein in ST2 cells, also resulted in a decrease in the density of the phospho-mTOR-band, suggesting that the suppression of the phospho-mTOR pathway is necessary for OPG production in ST2 cells. Thus, suitable suppression of mTOR phosphorylation is a necessary requirement for OPG production in bone marrow stromal cells.
巻・号 384(1)
ページ 82-6
公開日 2009-6-19
DOI 10.1016/j.bbrc.2009.04.084
PII S0006-291X(09)00776-1
PMID 19393224
MeSH Animals Bone Marrow Cells / drug effects Bone Marrow Cells / metabolism* Carrier Proteins / antagonists & inhibitors Carrier Proteins / metabolism* Cell Line Down-Regulation Mice Osteoprotegerin / biosynthesis* Phosphorylation Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors Phosphotransferases (Alcohol Group Acceptor) / metabolism* Sirolimus / pharmacology TOR Serine-Threonine Kinases Up-Regulation
IF 2.985
引用数 20
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224)