RRC ID 2194
Author Yamashita A, Sato M, Fujita A, Yamamoto M, Toda T.
Title The roles of fission yeast ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling.
Journal Mol. Biol. Cell
Abstract The Ase1/Prc1 proteins constitute a conserved microtubule-associated protein family that is implicated in central spindle formation and cytokinesis. Here we characterize a role for fission yeast Ase1. Ase1 localizes to microtubule overlapping zones and displays dynamic alterations of localization during the cell cycle. In particular, its spindle localization during metaphase is reduced substantially, followed by robust appearance at the spindle midzone in anaphase. ase1 deletions are viable but defective in nuclear and septum positioning and completion of cytokinesis, which leads to diploidization and chromosome loss. Time-lapse imaging shows that elongating spindles collapse abruptly in the middle of anaphase B. Either absence or overproduction of Ase1 results in profound defects on microtubule bundling in an opposed manner, indicating that Ase1 is a dose-dependent microtubule-bundling factor. In contrast microtubule nucleating activities are not noticeably compromised in ase1 mutants. During meiosis astral microtubules are not bundled and oscillatory nuclear movement is impaired significantly. The Aurora kinase does not correctly localize to central spindles in the absence of Ase1. Finally Ase1 acts as a regulatory component in the cytokinesis checkpoint that operates to inhibit nuclear division when the cytokinesis apparatus is perturbed. Ase1, therefore, couples anaphase completion with cytokinesis upon cell division.
Volume 16(3)
Pages 1378-95
Published 2005-3
DOI 10.1091/mbc.e04-10-0859
PII E04-10-0859
PMID 15647375
PMC PMC551500
MeSH Actomyosin / chemistry Anaphase Aurora Kinases Cell Division Cell Nucleus / metabolism* Cell Survival Cytokinesis Cytoplasm / metabolism Dose-Response Relationship, Drug Fluorescent Antibody Technique, Indirect Genome Genotype Green Fluorescent Proteins / metabolism Humans Image Processing, Computer-Assisted Interphase Kinetochores / metabolism Luminescent Proteins / metabolism Meiosis Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / physiology* Microtubules / metabolism Mitosis* Models, Genetic Mutation Oscillometry Plasmids / metabolism Polymerase Chain Reaction Protein Structure, Tertiary Protein-Serine-Threonine Kinases / metabolism Saccharomyces cerevisiae Proteins / physiology* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / physiology* Signal Transduction Spindle Apparatus / metabolism Time Factors
IF 3.512
Times Cited 81
Yeast FY13648(JW819) FY14415(HM248)