RRC ID 2241
Author He Y, Sugiura R, Ma Y, Kita A, Deng L, Takegawa K, Matsuoka K, Shuntoh H, Kuno T.
Title Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway.
Journal Genes Cells
Abstract We have previously isolated ypt3-i5 mutant and showed that Ypt3 GTPase functions in the fission yeast secretory pathway. Here, the same genetic screen led to the isolation of ryh1-i6, a mutant allele of the ryh1+ gene encoding a homolog of Rab6. The ryh1-i6 mutant showed phenotypes that support its role in retrograde traffic from endosome to the Golgi. Interestingly, ryh1+ gene deletion was synthetically lethal with ypt3-i5 mutation. Consistently, the over-expression of the GDP-conformational mutant, Ryh1T25 N, inhibited the growth of ypt3-i5 mutant but had no effect on that of wild-type cells. Furthermore, the over-expression of the Ryh1T25N mutant inhibited the acid phosphatase glycosylation and exacerbated the cell wall integrity of ypt3-i5 mutant, but had no effect on those of wild-type cells. GFP-Ryh1 and GFP-Ypt3 both localized at the Golgi/endosome, but showed distinct subcellular localizations. The localization of GFP-Ryh1 in ypt3-i5 mutant and that of GFP-Ypt3 in ryh1-i6 mutant were distinct from those in wild-type cells. In addition, Ryh1 as well as Ypt3 were shown to be involved in acid phosphatase secretion. These results suggest that Ryh1 is involved in the secretory pathway and may have a potential overlapping function with Ypt3 in addition to its role in recycling.
Volume 11(3)
Pages 207-21
Published 2006-3-1
DOI 10.1111/j.1365-2443.2006.00935.x
PMID 16483310
MeSH Acid Phosphatase / metabolism Amino Acid Sequence Cell Wall / metabolism Endosomes / metabolism Exocytosis / physiology* Glycosylation Golgi Apparatus / metabolism Molecular Sequence Data Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism* Mutagenesis, Site-Directed Mutation / genetics Phenotype Schizosaccharomyces / genetics Schizosaccharomyces / physiology* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Subcellular Fractions rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism*
IF 1.655
Times Cited 19
Yeast HM123