RRC ID 2862
Author Nakatani Y, Nishidate M, Fujita M, Kawakami A, Kudo A.
Title Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration.
Journal Dev. Growth Differ.
Abstract Urodeles and fish have higher regeneration ability in a variety of tissues and organs than do other vertebrate species including mammals. Though many studies have aimed at identifying the cellular and molecular basis for regeneration, relatively little is known about the detailed cellular behaviors and involved molecular basis. In the present study, a small molecule inhibitor was used to analyzed the role of phosphoinositide 3-kinase (PI3K) signaling during regeneration. We showed that the inhibitor disrupted the formation of blastema including the expression of characteristic genes. The failure of blastema formation was due to the impaired migration of mesenchymal cells to the distal prospective blastema region, although it had a little affect on cell cycle activation in mesenchymal cells. Moreover, we found that the epidermal remodeling including cell proliferation, distal cell migration and Akt phosphorylation was also affected by the inhibitor, implying a possible involvement of epidermis for proper formation of blastema. From these data, we propose a model in which distinct signals that direct the cell cycle activation, mesenchymal cell migration and epidermal remodeling coordinate together to accomplish the correct blastema formation and regeneration.
Volume 50(2)
Pages 71-83
Published 2008-2
DOI 10.1111/j.1440-169X.2007.00977.x
PMID 18042247
MeSH Androstadienes / pharmacology Animals Animals, Genetically Modified Blotting, Western Cell Movement* Chromones / pharmacology Enzyme Inhibitors / pharmacology Immunochemistry In Situ Hybridization Mesenchymal Stem Cells / cytology* Microscopy, Confocal Morpholines / pharmacology Neovascularization, Physiologic Oryzias / anatomy & histology Oryzias / physiology* Phosphatidylinositol 3-Kinases / antagonists & inhibitors Phosphatidylinositol 3-Kinases / metabolism Regeneration* Signal Transduction Wortmannin
IF 1.496
Times Cited 10
Medaka OK-Cab (MT830) Qurt(MT828)