RRC ID 30204
Author Ning S, Wang N, Sakuma S, Pourkheirandish M, Wu J, Matsumoto T, Koba T, Komatsuda T.
Title Structure, transcription and post-transcriptional regulation of the bread wheat orthologs of the barley cleistogamy gene Cly1.
Journal Theor Appl Genet
Abstract The majority of genes present in the hexaploid bread wheat genome are present as three homoeologs. Here, we describe the three homoeologous orthologs of the barley cleistogamy gene Cly1, a member of the AP2 gene family. As in barley, the wheat genes (designated TaAP2-A, -B and -D) map to the sub-telomeric region of the long arms of the group 2 chromosomes. The structure and pattern of transcription of the TaAP2 homoeologs were similar to those of Cly1. Transcript abundance was high in the florets, and particularly in the lodicule. The TaAP2 message was cleaved at its miR172 target sites. The set of homoeolog-specific PCR assays developed will be informative for identifying either naturally occurring or induced cleistogamous alleles at each of the three wheat homoeologs. By combining such alleles via conventional crossing, it should be possible to generate a cleistogamous form of bread wheat, which would be advantageous both with respect to improving the level of the crop's resistance against the causative pathogen of fusarium head blight, and for controlling pollen-mediated gene flow to and from genetically modified cultivars.
Volume 126(5)
Pages 1273-83
Published 2013-5-1
DOI 10.1007/s00122-013-2052-6
PMID 23381807
MeSH Alleles Amino Acid Sequence Base Sequence Biomarkers / metabolism Chromosomes, Artificial, Bacterial Chromosomes, Plant / chemistry Chromosomes, Plant / genetics* Flowers / genetics* Gene Expression Profiling Gene Expression Regulation, Plant* Genome, Plant Hordeum / genetics* Hordeum / growth & development Hordeum / metabolism MicroRNAs / genetics Molecular Sequence Data Oligonucleotide Array Sequence Analysis Phylogeny Plant Proteins / genetics* Plant Proteins / metabolism RNA Processing, Post-Transcriptional RNA, Messenger / genetics RNA, Plant / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Triticum / genetics* Triticum / growth & development Triticum / metabolism
IF 4.439
Times Cited 16