論文 - 詳細
| RRC ID | 30214 |
|---|---|
| 著者 | Yokoo R, Zawadzki KA, Nabeshima K, Drake M, Arur S, Villeneuve AM. |
| タイトル | COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers. |
| ジャーナル | Cell |
| Abstract |
Crossovers (COs) between homologous chromosomes ensure their faithful segregation during meiosis. We identify C. elegans COSA-1, a cyclin-related protein conserved in metazoa, as a key component required to convert meiotic double-strand breaks (DSBs) into COs. During late meiotic prophase, COSA-1 localizes to foci that correspond to the single CO site on each homolog pair and indicate sites of eventual concentration of other conserved CO proteins. Chromosomes gain and lose competence to load CO proteins during meiotic progression, with competence to load COSA-1 requiring prior licensing. Our data further suggest a self-reinforcing mechanism maintaining CO designation. Modeling of a nonlinear dose-response relationship between IR-induced DSBs and COSA-1 foci reveals efficient conversion of DSBs into COs when DSBs are limiting and a robust capacity to limit cytologically differentiated CO sites when DSBs are in excess. COSA-1 foci serve as a unique live cell readout for investigating CO formation and CO interference. |
| 巻・号 | 149(1) |
| ページ | 75-87 |
| 公開日 | 2012-3-30 |
| DOI | 10.1016/j.cell.2012.01.052 |
| PII | S0092-8674(12)00286-3 |
| PMID | 22464324 |
| PMC | PMC3339199 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosomes / metabolism Crossing Over, Genetic* Cyclins / genetics Cyclins / metabolism* DNA Breaks, Double-Stranded DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Meiosis* Models, Molecular Mutation |
| IF | 38.637 |
| 引用数 | 105 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3298 |