論文 - 詳細
| RRC ID | 30811 |
|---|---|
| 著者 | Mosallanejad K, Sekine Y, Ishikura-Kinoshita S, Kumagai K, Nagano T, Matsuzawa A, Takeda K, Naguro I, Ichijo H. |
| タイトル | The DEAH-box RNA helicase DHX15 activates NF-κB and MAPK signaling downstream of MAVS during antiviral responses. |
| ジャーナル | Sci Signal |
| Abstract |
During infection with an RNA virus, the DExD/H-box RNA helicases RIG-I (retinoic acid-inducible gene I) and MDA5 (melanoma differentiation-associated gene 5) activate the interferon regulatory factor 3 (IRF3), nuclear factor κB (NF-κB), c-Jun amino-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) signaling pathways through an unknown mechanism involving the adaptor protein MAVS (mitochondrial antiviral signaling). We used a Drosophila misexpression screen to identify DEAH-box polypeptide 15 (DHX15) as an activator of the p38 MAPK pathway. Human DHX15 contributed to the activation of the NF-κB, JNK, and p38 MAPK pathways, but not the IRF3 pathway, in response to the synthetic double-stranded RNA analog poly(I:C) (polyinosinic-polycytidylic acid), and DHX15 was required for optimal cytokine production in response to poly(I:C) and infection with RNA virus. DHX15 physically interacted with MAVS and mediated the MAVS-dependent activation of the NF-κB and MAPK pathways. Furthermore, DHX15 was required for poly(I:C)- and RNA virus-dependent, MAVS-mediated apoptosis. Thus, our findings indicate that, in RIG-I-like receptor signaling, DHX15 specifically stimulates the NF-κB and MAPK pathways downstream of MAVS and contributes to MAVS-mediated cytokine production and apoptosis. |
| 巻・号 | 7(323) |
| ページ | ra40 |
| 公開日 | 2014-4-29 |
| DOI | 10.1126/scisignal.2004841 |
| PII | 7/323/ra40 |
| PMID | 24782566 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Apoptosis / genetics Blotting, Western Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Encephalomyocarditis virus / physiology HEK293 Cells HeLa Cells Host-Pathogen Interactions Humans Interferon-beta / genetics Interferon-beta / metabolism Interleukin-6 / genetics Interleukin-6 / metabolism JNK Mitogen-Activated Protein Kinases MAP Kinase Signaling System* Mutation NF-kappa B / metabolism* Poly I-C / genetics RNA Helicases / genetics RNA Helicases / metabolism* RNA Interference Reverse Transcriptase Polymerase Chain Reaction Sendai virus / physiology TNF Receptor-Associated Factor 6 / genetics TNF Receptor-Associated Factor 6 / metabolism Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / metabolism |
| IF | 6.467 |
| 引用数 | 37 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 11107R-2 |