論文 - 詳細
| RRC ID | 31340 |
|---|---|
| 著者 | Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R. |
| タイトル | Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. |
| ジャーナル | Science |
| Abstract |
The basic helix-loop-helix transcription factors Ascl1/Mash1, Hes1, and Olig2 regulate fate choice of neurons, astrocytes, and oligodendrocytes, respectively. These same factors are coexpressed by neural progenitor cells. Here, we found by time-lapse imaging that these factors are expressed in an oscillatory manner by mouse neural progenitor cells. In each differentiation lineage, one of the factors becomes dominant. We used optogenetics to control expression of Ascl1 and found that, although sustained Ascl1 expression promotes neuronal fate determination, oscillatory Ascl1 expression maintains proliferating neural progenitor cells. Thus, the multipotent state correlates with oscillatory expression of several fate-determination factors, whereas the differentiated state correlates with sustained expression of a single factor. |
| 巻・号 | 342(6163) |
| ページ | 1203-8 |
| 公開日 | 2013-12-6 |
| DOI | 10.1126/science.1242366 |
| PII | science.1242366 |
| PMID | 24179156 |
| MeSH | Animals Astrocytes / cytology Astrocytes / metabolism Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism* Cell Lineage Cell Proliferation Female Gene Knock-In Techniques Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Male Mice Mice, Transgenic Multipotent Stem Cells / physiology* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neural Stem Cells / physiology* Neurogenesis* Neurons / cytology Neurons / metabolism Oligodendrocyte Transcription Factor 2 Oligodendroglia / cytology Oligodendroglia / metabolism Optogenetics Telencephalon / cytology Telencephalon / metabolism Transcription Factor HES-1 Up-Regulation |
| IF | 41.846 |
| 引用数 | 241 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| 実験動物マウス | RBRC02892 |