論文 - 詳細
| RRC ID | 32683 |
|---|---|
| 著者 | Crespo CL, Vernieri C, Keller PJ, Garrè M, Bender JR, Wittbrodt J, Pardi R. |
| タイトル | The PAR complex controls the spatiotemporal dynamics of F-actin and the MTOC in directionally migrating leukocytes. |
| ジャーナル | J Cell Sci |
| Abstract |
Inflammatory cells acquire a polarized phenotype to migrate towards sites of infection or injury. A conserved polarity complex comprising PAR-3, PAR-6 and atypical protein kinase C (aPKC) relays extracellular polarizing cues to control cytoskeletal and signaling networks affecting morphological and functional polarization. However, there is no evidence that myeloid cells use PAR signaling to migrate vectorially in three-dimensional (3D) environments in vivo. Using genetically encoded bioprobes and high-resolution live imaging, we reveal the existence of F-actin oscillations in the trailing edge and constant repositioning of the microtubule organizing center (MTOC) to direct leukocyte migration in wounded medaka fish larvae (Oryzias latipes). Genetic manipulation in live myeloid cells demonstrates that the catalytic activity of aPKC and the regulated interaction with PAR-3 and PAR-6 are required for consistent F-actin oscillations, MTOC perinuclear mobility, aPKC repositioning and wound-directed migration upstream of Rho kinase (also known as ROCK or ROK) activation. We propose that the PAR complex coordinately controls cytoskeletal changes affecting both the generation of traction force and the directionality of leukocyte migration to sites of injury. |
| 巻・号 | 127(Pt 20) |
| ページ | 4381-95 |
| 公開日 | 2014-10-15 |
| DOI | 10.1242/jcs.146217 |
| PII | jcs.146217 |
| PMID | 25179599 |
| PMC | PMC4197085 |
| MeSH | Actin Cytoskeleton / metabolism Actins / metabolism Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Animals, Genetically Modified Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Movement* Cell Polarity / genetics Cells, Cultured Leukocytes / physiology* Microtubule-Organizing Center / physiology* Multiprotein Complexes / genetics Mutation / genetics Oryzias Protein Kinase C / genetics Protein Kinase C / metabolism* Protein Transport Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* rho-Associated Kinases / metabolism |
| IF | 4.573 |
| 引用数 | 14 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | TG(FmpoP::EB3-EGFP/FmpoP::RFP-Lifeact) (TG1043) TG(FmpoP::mCherry) (TG1044) |