論文 - 詳細
| RRC ID | 33279 |
|---|---|
| 著者 | Kaneko T, Sakuma T, Yamamoto T, Mashimo T. |
| タイトル | Simple knockout by electroporation of engineered endonucleases into intact rat embryos. |
| ジャーナル | Sci Rep |
| Abstract |
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the microinjection of endonucleases into embryos requires a high skill level, is time consuming, and may cause damage to embryos. Here, we demonstrate that the electroporation of endonuclease mRNAs into intact embryos can induce editing at targeted loci and efficiently produce knockout rats. It is noteworthy that the electroporation of ZFNs resulted in an embryonic survival rate (91%) and a genome-editing rate (73%) that were more than 2-fold higher than the corresponding rates from conventional microinjection. Electroporation technology provides a simple and effective method to produce knockout animals. |
| 巻・号 | 4 |
| ページ | 6382 |
| 公開日 | 2014-10-1 |
| DOI | 10.1038/srep06382 |
| PII | srep06382 |
| PMID | 25269785 |
| PMC | PMC4180828 |
| MeSH | Animals CRISPR-Cas Systems* Cells, Cultured Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Electroporation / methods* Embryo Transfer Embryo, Mammalian / cytology Embryo, Mammalian / metabolism* Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Female Gene Targeting / methods* Genetic Engineering / methods* Male Rats Rats, Inbred F344 Zinc Fingers |
| IF | 3.998 |
| 引用数 | 82 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 33 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | F344/Stm(strainID=266) |
| ヒト・動物細胞 | Rat-1(RCB1830) |