Reference - Detail
| RRC ID | 35326 |
|---|---|
| Author | Persson O, Nyström T, Farewell A. |
| Title | UspB, a member of the sigma-S regulon, facilitates RuvC resolvase function. |
| Journal | DNA Repair (Amst) |
| Abstract |
A growing body of evidence shows that there is an intimate connection between proteins required for genome stability and stationary phase survival. In this work we show that the integral membrane protein UspB, a member of the RpoS regulon, is required for proper DNA repair as mutants lacking uspB are hypersensitive to several DNA damaging agents including ultraviolet light, mitomycin C, bleomycin and ciprofloxacin. Genetic and physical studies demonstrate that UspB acts in the RuvABC recombination repair pathway and removing uspB creates a phenocopy of the Holliday junction resolvase mutant, ruvC. Further, we show that the uspB mutant phenotype can be suppressed by ectopic overproduction of RuvC and that both ruvC and uspB mutants can be suppressed by inactivating recD. The fact that RuvABC-dependent repair requires UspB for proper activity suggests that the sigma-S regulon works together with DNA repair pathways under stress conditions to defend the cell against genotoxic stress. |
| Volume | 9(11) |
| Pages | 1162-9 |
| Published | 2010-11-10 |
| DOI | 10.1016/j.dnarep.2010.08.004 |
| PII | S1568-7864(10)00270-3 |
| PMID | 20817623 |
| MeSH | Bacterial Proteins / genetics* Bacterial Proteins / metabolism DNA Repair / genetics DNA Repair / radiation effects Endodeoxyribonucleases / biosynthesis Endodeoxyribonucleases / metabolism* Escherichia coli K12 / genetics* Escherichia coli K12 / metabolism* Escherichia coli K12 / radiation effects Escherichia coli Proteins / biosynthesis Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial / radiation effects Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Phenotype Recombination, Genetic Regulon* Sigma Factor / genetics* Sigma Factor / metabolism Ultraviolet Rays |
| IF | 3.339 |
| Times Cited | 1 |
| WOS Category | GENETICS & HEREDITY TOXICOLOGY |
| Resource | |
| Prokaryotes E. coli | JW1852? JW3672? |