論文 - 詳細
| RRC ID | 35674 |
|---|---|
| 著者 | Kusano K, Johnson-Schlitz DM, Engels WR. |
| タイトル | Sterility of Drosophila with mutations in the Bloom syndrome gene--complementation by Ku70. |
| ジャーナル | Science |
| Abstract |
The Drosophila Dmblm locus is a homolog of the human Bloom syndrome gene, which encodes a helicase of the RECQ family. We show that Dmblm is identical to mus309, a locus originally identified in a mutagen-sensitivity screen. One mus309 allele, which carries a stop codon between two of the helicase motifs, causes partial male sterility and complete female sterility. Mutant males produce an excess of XY sperm and nullo sperm, consistent with a high frequency of nondisjunction and/or chromosome loss. These phenotypes of mus309 suggest that Dmblm functions in DNA double-strand break repair. The mutant Dmblm phenotypes were partially rescued by an extra copy of the DNA repair gene Ku70, indicating that the two genes functionally interact in vivo. |
| 巻・号 | 291(5513) |
| ページ | 2600-2 |
| 公開日 | 2001-3-30 |
| DOI | 10.1126/science.291.5513.2600 |
| PII | 291/5513/2600 |
| PMID | 11283371 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / physiology Alleles Amino Acid Substitution Animals Antigens, Nuclear* Bloom Syndrome / genetics Chromosome Breakage DNA Damage DNA Helicases / genetics* DNA Helicases / physiology* DNA Repair DNA-Binding Proteins / genetics* DNA-Binding Proteins / physiology Drosophila melanogaster / enzymology Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female Fertility Genes, Insect Genetic Complementation Test Genetic Markers Ku Autoantigen Male Mutagenesis, Insertional Mutation Nondisjunction, Genetic Nuclear Proteins / genetics* Nuclear Proteins / physiology Phenotype RecQ Helicases Recombination, Genetic Sequence Deletion Spermatozoa / physiology Transgenes Y Chromosome / genetics |
| IF | 41.846 |
| 引用数 | 87 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| ショウジョウバエ | |