論文 - 詳細
| RRC ID | 35680 |
|---|---|
| 著者 | Matsuhara H, Yamamoto A. |
| タイトル | Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast. |
| ジャーナル | Genes Cells |
| Abstract |
Autophagy is a conserved intracellular degradation system, which contributes to development and differentiation of various organisms. Yeast cells undergo meiosis under nitrogen-starved conditions and require autophagy for meiosis initiation. However, the precise roles of autophagy in meiosis remain unclear. Here, we show that autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast. Autophagy-defective strains bearing a mutation in the autophagy core factor gene atg1, atg7, or atg14 exhibit deformed nuclear structures during meiosis. These mutant cells require an extracellular nitrogen supply for meiosis progression following their entry into meiosis and show delayed meiosis progression even with a nitrogen supply. In addition, they show frequent chromosome dissociation from the spindle together with spindle overextension, forming extra nuclei. Furthermore, Aurora kinase, which regulates chromosome segregation and spindle elongation, is significantly increased at the centromere and spindle in the mutant cells. Aurora kinase down-regulation eliminated delayed initiation of meiosis I and II, chromosome dissociation, and spindle overextension, indicating that increased Aurora kinase activity may cause these aberrances in the mutant cells. Our findings show a hitherto unrecognized relationship of autophagy with the nuclear structure, regulation of cell cycle progression, and chromosome segregation in meiosis. |
| 巻・号 | 21(1) |
| ページ | 65-87 |
| 公開日 | 2016-1-1 |
| DOI | 10.1111/gtc.12320 |
| PMID | 26696398 |
| MeSH | Anaphase / drug effects Aurora Kinases / metabolism Autophagy* / drug effects Autophagy* / genetics Cell Nucleus / drug effects Cell Nucleus / pathology Centromere / drug effects Centromere / metabolism Chromosome Segregation* / drug effects Cloning, Molecular Down-Regulation / drug effects Down-Regulation / genetics Genes, Insect Intracellular Space / metabolism M Phase Cell Cycle Checkpoints / drug effects Meiosis* / drug effects Meiosis* / genetics Mutation / genetics Nitrogen / pharmacology Phenotype Schizosaccharomyces / cytology* Schizosaccharomyces / drug effects Schizosaccharomyces / enzymology Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / metabolism Spindle Apparatus / drug effects Spindle Apparatus / metabolism Spores, Fungal / drug effects Spores, Fungal / physiology |
| IF | 1.655 |
| 引用数 | 7 |
| WOS 分野 | CELL BIOLOGY GENETICS & HEREDITY |
| リソース情報 | |
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