Reference - Detail
RRC ID | 3603 |
---|---|
Author | Matsubara Y, Kawasaki I, Urushiyama S, Yasuda T, Shirakata M, Iino Y, Shibuya H, Yamanashi Y. |
Title | The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans. |
Journal | Genes Cells |
Abstract |
The Ras-MAP kinase pathway regulates varieties of fundamental cellular events. In Caenorhabditis elegans, this pathway is required for oocyte development; however, the nature of its up-stream regulators has remained elusive. Here, we identified a C. elegans gene, rog-1, which encodes the only protein having the IRS-type phosphotyrosine-binding (PTB) domain in the worms. ROG-1 has no obvious domain structure aside from the PTB domain, suggesting that it could serve as an adaptor down-stream of protein-tyrosine kinases (PTKs). RNA interference (RNAi)-mediated down-regulation of rog-1 mRNA significantly decreased brood size. rog-1(tm1031) truncation mutants showed a severe disruption in progression of developing oocytes from pachytene to diakinesis, as was seen in worms carrying a loss-of-function mutation in the let-60 Ras or mpk-1 MAP kinase gene. Furthermore, let-60 Ras-regulated activation of MPK-1 in the gonad is undetectable in rog-1(tm1031) mutants. Conversely, a gain-of-function mutation in the let-60 Ras gene rescues the brood size reduction and germ cell abnormality in rog-1(tm1031) worms. Consistently, rog-1 is preferentially expressed in the germ cells and its expression in the gonad is essential for oocyte development. Thus, ROG-1 is a key positive regulator of the Ras-MAP kinase pathway that permits germ cells to exit from pachytene. |
Volume | 12(3) |
Pages | 407-20 |
Published | 2007-3-1 |
DOI | 10.1111/j.1365-2443.2007.01061.x |
PII | GTC1061 |
PMID | 17352744 |
MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA, Helminth / genetics Female Genes, Helminth MAP Kinase Signaling System* Male Meiosis / genetics Meiosis / physiology* Mitogen-Activated Protein Kinase 1 Molecular Sequence Data Mutation Oogenesis Phylogeny Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology, Amino Acid ras Proteins / genetics ras Proteins / metabolism* |
IF | 1.655 |
Times Cited | 4 |
WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
Resource | |
C.elegans | tm1031 |