RRC ID |
36261
|
著者 |
Iwanami N, Nakamura Y, Satoh T, Liu Z, Satoh AK.
|
タイトル |
Rab6 Is Required for Multiple Apical Transport Pathways but Not the Basolateral Transport Pathway in Drosophila Photoreceptors.
|
ジャーナル |
PLoS Genet
|
Abstract |
Polarized membrane trafficking is essential for the construction and maintenance of multiple plasma membrane domains of cells. Highly polarized Drosophila photoreceptors are an excellent model for studying polarized transport. A single cross-section of Drosophila retina contains many photoreceptors with 3 clearly differentiated plasma membrane domains: a rhabdomere, stalk, and basolateral membrane. Genome-wide high-throughput ethyl methanesulfonate screening followed by precise immunohistochemical analysis identified a mutant with a rare phenotype characterized by a loss of 2 apical transport pathways with normal basolateral transport. Rapid gene identification using whole-genome resequencing and single nucleotide polymorphism mapping identified a nonsense mutation of Rab6 responsible for the apical-specific transport deficiency. Detailed analysis of the trafficking of a major rhabdomere protein Rh1 using blue light-induced chromophore supply identified Rab6 as essential for Rh1 to exit the Golgi units. Rab6 is mostly distributed from the trans-Golgi network to a Golgi-associated Rab11-positive compartment that likely recycles endosomes or transport vesicles going to recycling endosomes. Furthermore, the Rab6 effector, Rich, is required for Rab6 recruitment in the trans-Golgi network. Moreover, a Rich null mutation phenocopies the Rab6 null mutant, indicating that Rich functions as a guanine nucleotide exchange factor for Rab6. The results collectively indicate that Rab6 and Rich are essential for the trans-Golgi network-recycling endosome transport of cargoes destined for 2 apical domains. However, basolateral cargos are sorted and exported from the trans-Golgi network in a Rab6-independent manner.
|
巻・号 |
12(2)
|
ページ |
e1005828
|
公開日 |
2016-2-1
|
DOI |
10.1371/journal.pgen.1005828
|
PII |
PGENETICS-D-15-01927
|
PMID |
26890939
|
PMC |
PMC4758697
|
MeSH |
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism
Animals
Animals, Genetically Modified
Drosophila / drug effects
Drosophila / genetics
Drosophila / metabolism*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Endosomes / metabolism
Ethyl Methanesulfonate / pharmacology
Golgi Apparatus / metabolism*
Membrane Proteins / genetics
Membrane Proteins / metabolism
Mutagenesis
Mutation
Photoreceptor Cells, Invertebrate / metabolism*
Protein Transport
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism*
|
IF |
5.175
|
引用数 |
20
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
ショウジョウバエ |
|