論文 - 詳細
| RRC ID | 36261 |
|---|---|
| 著者 | Iwanami N, Nakamura Y, Satoh T, Liu Z, Satoh AK. |
| タイトル | Rab6 Is Required for Multiple Apical Transport Pathways but Not the Basolateral Transport Pathway in Drosophila Photoreceptors. |
| ジャーナル | PLoS Genet |
| Abstract |
Polarized membrane trafficking is essential for the construction and maintenance of multiple plasma membrane domains of cells. Highly polarized Drosophila photoreceptors are an excellent model for studying polarized transport. A single cross-section of Drosophila retina contains many photoreceptors with 3 clearly differentiated plasma membrane domains: a rhabdomere, stalk, and basolateral membrane. Genome-wide high-throughput ethyl methanesulfonate screening followed by precise immunohistochemical analysis identified a mutant with a rare phenotype characterized by a loss of 2 apical transport pathways with normal basolateral transport. Rapid gene identification using whole-genome resequencing and single nucleotide polymorphism mapping identified a nonsense mutation of Rab6 responsible for the apical-specific transport deficiency. Detailed analysis of the trafficking of a major rhabdomere protein Rh1 using blue light-induced chromophore supply identified Rab6 as essential for Rh1 to exit the Golgi units. Rab6 is mostly distributed from the trans-Golgi network to a Golgi-associated Rab11-positive compartment that likely recycles endosomes or transport vesicles going to recycling endosomes. Furthermore, the Rab6 effector, Rich, is required for Rab6 recruitment in the trans-Golgi network. Moreover, a Rich null mutation phenocopies the Rab6 null mutant, indicating that Rich functions as a guanine nucleotide exchange factor for Rab6. The results collectively indicate that Rab6 and Rich are essential for the trans-Golgi network-recycling endosome transport of cargoes destined for 2 apical domains. However, basolateral cargos are sorted and exported from the trans-Golgi network in a Rab6-independent manner. |
| 巻・号 | 12(2) |
| ページ | e1005828 |
| 公開日 | 2016-2-1 |
| DOI | 10.1371/journal.pgen.1005828 |
| PII | PGENETICS-D-15-01927 |
| PMID | 26890939 |
| PMC | PMC4758697 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Animals Animals, Genetically Modified Drosophila / drug effects Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Endosomes / metabolism Ethyl Methanesulfonate / pharmacology Golgi Apparatus / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Mutagenesis Mutation Photoreceptor Cells, Invertebrate / metabolism* Protein Transport rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* |
| IF | 5.175 |
| 引用数 | 20 |
| WOS 分野 | GENETICS & HEREDITY |
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