| RRC ID |
3627
|
| 著者 |
Sapir A, Choi J, Leikina E, Avinoam O, Valansi C, Chernomordik LV, Newman AP, Podbilewicz B.
|
| タイトル |
AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Cell fusion is fundamental for reproduction and organ formation. Fusion between most C. elegans epithelial cells is mediated by the EFF-1 fusogen. However, fusion between the anchor cell and the utse syncytium that establishes a continuous uterine-vulval tube proceeds normally in eff-1 mutants. By isolating mutants where the anchor-cell fails to fuse, we identified aff-1. AFF-1 ectopic expression results in fusion of cells that normally do not fuse in C. elegans. The fusogen activity of AFF-1 was further confirmed by its ability to fuse heterologous cells. AFF-1 and EFF-1 differ in their fusogenic activity and expression patterns but share eight conserved predicted disulfide bonds in their ectodomains, including a putative TGF-beta-type-I-Receptor domain. We found that FOS-1, the Fos transcription factor ortholog that controls anchor-cell invasion during nematode development, is a specific activator of aff-1-mediated anchor-cell fusion. Thus, FOS-1 links cell invasion and fusion in a developmental cascade.
|
| 巻・号 |
12(5)
|
| ページ |
683-98
|
| 公開日 |
2007-5-1
|
| DOI |
10.1016/j.devcel.2007.03.003
|
| PII |
S1534-5807(07)00103-7
|
| PMID |
17488621
|
| PMC |
PMC1975806
|
| MeSH |
Amino Acid Sequence
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / embryology
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism*
Cell Fusion
Cytoplasm / metabolism
Embryo, Nonmammalian / cytology
Epithelial Cells / cytology
Female
Insecta / cytology
Models, Biological
Molecular Sequence Data
Mutation / genetics
Phenotype
Proto-Oncogene Proteins c-fos / chemistry
Proto-Oncogene Proteins c-fos / metabolism*
Transcription Factors / chemistry
Transcription Factors / metabolism*
Vulva / cytology
Vulva / growth & development
|
| IF |
10.092
|
| 引用数 |
71
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2214 |
