RRC ID 36344
Author Takamatsu K, Iehisa JC, Nishijima R, Takumi S.
Title Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives.
Journal Plant Mol Biol
Abstract Hybrid necrosis is a well-known reproductive isolation mechanism in plant species, and an autoimmune response is generally considered to trigger hybrid necrosis through epistatic interaction between disease resistance-related genes in hybrids. In common wheat, the complementary Ne1 and Ne2 genes control hybrid necrosis, defined as type I necrosis. Two other types of hybrid necrosis (type II and type III) have been observed in interspecific hybrids between tetraploid wheat and Aegilops tauschii. Another type of hybrid necrosis, defined here as type IV necrosis, has been reported in F1 hybrids between Triticum urartu and some accessions of Triticum monococcum ssp. aegilopoides. In types I, III and IV, cell death occurs gradually starting in older tissues, whereas type II necrosis symptoms occur only under low temperature. To compare comprehensive gene expression patterns of hybrids showing growth abnormalities, transcriptome analysis of type I and type IV necrosis was performed using a wheat 38k oligo-DNA microarray. Defense-related genes including many WRKY transcription factor genes were dramatically up-regulated in plants showing type I and type IV necrosis, similarly to other known hybrid abnormalities, suggesting an association with an autoimmune response. Reactive oxygen species generation and necrotic cell death were effectively inhibited by ZnCl2 treatment in types I, III and IV necrosis, suggesting a significant association of Ca(2+) influx in upstream signaling of necrotic cell death in wheat hybrid necrosis.
Volume 88(4-5)
Pages 487-502
Published 2015-7-1
DOI 10.1007/s11103-015-0338-6
PMID 26081164
MeSH Calcium Signaling Cell Death / drug effects Cell Death / genetics Chlorides / pharmacology Genes, Plant Hybridization, Genetic* Oligonucleotide Array Sequence Analysis Phenotype Photosynthesis Poaceae / drug effects Poaceae / genetics Poaceae / growth & development Polyploidy RNA, Plant / genetics Reactive Oxygen Species / metabolism Species Specificity Transcriptome Triticum / drug effects Triticum / genetics* Triticum / growth & development Zinc Compounds / pharmacology
IF 3.302
Times Cited 6
Wheat KU-101-3 KU-8001 KU-3620 KU-199-5 KU-199- 8 KU-199-9 KU-199-11 KU-199-16 KU-2059 KU-2828