RRC ID 39374
著者 Tominaga K, Morisaki H, Kaneko Y, Fujimoto A, Tanaka T, Ohtsubo M, Hirai M, Okayama H, Ikeda K, Nakanishi M.
タイトル Role of human Cds1 (Chk2) kinase in DNA damage checkpoint and its regulation by p53.
ジャーナル J Biol Chem
Abstract In response to DNA damage, mammalian cells adopt checkpoint regulation, by phosphorylation and stabilization of p53, to delay cell cycle progression. However, most cancer cells that lack functional p53 retain an unknown checkpoint mechanism(s) by which cells are arrested at the G(2)/M phase. Here we demonstrate that a human homolog of Cds1/Rad53 kinase (hCds1) is rapidly phosphorylated and activated in response to DNA damage not only in normal cells but in cancer cells lacking functional p53. A survey of various cancer cell lines revealed that the expression level of hCds1 mRNA is inversely related to the presence of functional p53. In addition, transfection of normal human fibroblasts with SV40 T antigen or human papilloma viruses E6 or E7 causes a marked induction of hCds1 mRNA, and the introduction of functional p53 into SV40 T antigen- and E6-, but not E7-, transfected cells decreases the hCds1 level, suggesting that p53 negatively regulates the expression of hCds1. In cells without functional ataxia telangiectasia mutated (ATM) protein, phosphorylation and activation of hCds1 were observed in response to DNA damage induced by UV but not by ionizing irradiation. These results suggest that hCds1 is activated through an ATM-dependent as well as -independent pathway and that it may complement the function of p53 in DNA damage checkpoints in mammalian cells.
巻・号 274(44)
ページ 31463-7
公開日 1999-10-29
DOI 10.1074/jbc.274.44.31463
PII S0021-9258(19)51642-8
PMID 10531348
MeSH Ataxia Telangiectasia Mutated Proteins Cell Compartmentation Cell Cycle* Cell Cycle Proteins Checkpoint Kinase 2 Chromosome Mapping Chromosomes, Human, Pair 22 Cloning, Molecular DNA Damage* DNA-Binding Proteins Enzyme Activation Humans Nuclear Proteins / metabolism Phosphorylation Protein Kinases / genetics Protein Kinases / metabolism* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Tumor Suppressor Protein p53 / metabolism* Tumor Suppressor Proteins
IF 4.238
引用数 76
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 WI-38