論文 - 詳細
| RRC ID | 39535 |
|---|---|
| 著者 | Fukumi S, Horiguchi-Yamada J, Nakada S, Nagai M, Ohno T, Yamada H. |
| タイトル | Differential responses of Bcl-2 family genes to etoposide in chronic myeloid leukemia K562 cells. |
| ジャーナル | Mol Cell Biochem |
| Abstract |
Etoposide is a potent anticancer agent that is used to treat various tumors. We have investigated the dose-dependent effect of etoposide on apoptosis using chronic myeloid leukemia K562 cells treated with low (5 microM) or high (100 microM) concentrations of the drug. At a low concentration, etoposide induced little apoptosis at 24 h, while about 20% of the cells showed apoptosis morphologically at a high concentration. Processing of caspase-3 was slightly detected from 12 h and became obvious at 24 h with 100 microM etoposide. Caspase-3-like protease activity was detected at 24 h with a high concentration. Moreover, these changes were accompanied by cleavage of poly ADP ribose polymerase (PARP). Changes of the mRNA levels of most apoptosis-regulating genes were not prominent at both concentrations, except for the rapid induction of c-IAP-2/HIAP-1 and the down-regulation of Bcl-X(L) by 100 microM etoposide. The downregulation of Bcl-X(L) protein occurred from 6 h, while Bax protein conversely showed a slight increase from 6 h. Taken together, the present findings show that the dose-dependent apoptotic effect of etoposide is based on a change in the balance between Bcl-X(L) and Bax, which precedes the activation of caspase-3. |
| 巻・号 | 206(1-2) |
| ページ | 43-50 |
| 公開日 | 2000-3-1 |
| DOI | 10.1023/a:1007056727876 |
| PMID | 10839193 |
| MeSH | Antineoplastic Agents, Phytogenic / pharmacology* Apoptosis / drug effects Caspase 3 Caspases / metabolism DNA Damage / drug effects DNA Primers / chemistry DNA, Neoplasm / drug effects Dose-Response Relationship, Drug Down-Regulation Etoposide / pharmacology* Gene Expression Regulation Genes, bcl-2 / drug effects* Humans Immunoblotting K562 Cells / drug effects* K562 Cells / metabolism Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism Microscopy, Fluorescence Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases Proteins / metabolism Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism* RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction bcl-2-Associated X Protein bcl-X Protein |
| IF | 2.795 |
| 引用数 | 17 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |