論文 - 詳細
| RRC ID | 39589 |
|---|---|
| 著者 | Fukuda M, Kuroda TS, Mikoshiba K. |
| タイトル | Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. |
| ジャーナル | J Biol Chem |
| Abstract |
Myosin Va is a member of the unconventional class V myosin family, and a mutation in the myosin Va gene causes pigment granule transport defects in human Griscelli syndrome and dilute mice. How myosin Va recognizes its cargo (i.e. melanosomes), however, has remained undetermined over the past decade. In this study, we discovered Slac2-a/melanophilin to be the "missing link" between myosin Va and GTP-Rab27A present in the melanosome. Deletion analysis and site-directed mutagenesis showed that the N-terminal Slp (synaptotagmin-like protein) homology domain of Slac2-a specifically binds Rab27A/B isoforms and that the C-terminal half directly binds the globular tail of myosin Va. The tripartite protein complex (Rab27A.Slac2-a.myosin Va) in melanoma cells was further confirmed by immunoprecipitation. The discovery that myosin Va indirectly recognizes its cargo through Slac2-a, a novel Rab27A/B effector, should shed light on molecular recognition of its specific cargo by class V myosin. |
| 巻・号 | 277(14) |
| ページ | 12432-6 |
| 公開日 | 2002-4-5 |
| DOI | 10.1074/jbc.C200005200 |
| PII | S0021-9258(18)52075-5 |
| PMID | 11856727 |
| MeSH | Adaptor Proteins, Signal Transducing Animals Brain / metabolism COS Cells Carrier Proteins / chemistry* Carrier Proteins / metabolism* Cloning, Molecular DNA, Complementary / metabolism Gene Deletion Humans Melanosomes / metabolism Mice Models, Biological Mutagenesis, Site-Directed Mutation Myosin Heavy Chains / chemistry Myosin Heavy Chains / metabolism* Myosin Type V / chemistry Myosin Type V / metabolism* Precipitin Tests Protein Binding Protein Isoforms Protein Structure, Tertiary Protein Transport Transfection rab GTP-Binding Proteins / chemistry rab GTP-Binding Proteins / metabolism* rab27 GTP-Binding Proteins |
| IF | 4.238 |
| 引用数 | 254 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Slac2-a (RDB03422) pEF-FLAG-mouse myosin Va (Brain type) (RDB15283) pEF-FLAG-mouse Rab34 (RDB15394) pEF-T7-GST (RDB19882) |