Reference - Detail
| RRC ID | 39759 |
|---|---|
| Author | Fuji Y, Matsura T, Kai M, Kawasaki H, Yamada K. |
| Title | Protection by polaprezinc, an anti-ulcer drug, against indomethacin-induced apoptosis in rat gastric mucosal cells. |
| Journal | Jpn J Pharmacol |
| Abstract |
Polaprezinc [N-(3-aminopropionyl)-L-histidinato zinc] (PZ), an anti-ulcer drug, is a chelate compound consisting of zinc and L-carnosine. PZ has been shown to prevent gastric mucosal injury. In the present study, we investigated the inhibitory effect of PZ on indomethacin (IND)-induced apoptosis in a rat gastric mucosal cell line, RGM1. Pretreatment with PZ suppressed caspase-3 activation and subsequent apoptosis in the cells exposed to 500 microM IND in a dose-dependent manner, and 50 microM PZ exhibited the maximum inhibitory effect. Among PZ subcomponents, zinc but not L-carnosine played a pivotal role in this antiapoptotic function. PZ did not affect mitochondrial cytochrome c release upstream of caspase-3 activation in the IND-induced apoptotic signal pathway. Treatment with 500 microM IND evidently produced reactive oxygen species (ROS) in RGM1 cells. However, PZ did not scavenge ROS in IND-treated cells. Moreover, N-acetylL-cysteine, a potent antioxidant, inhibited ROS generation but did not suppress apoptosis in RGM1 cells exposed to IND. These observations demonstrate a novel pharmacological action of PZ; i.e., that PZ, and in particular its zinc subcomponent, inhibits apoptosis via inhibition of caspase-3 activation but not antioxidant activity. |
| Volume | 84(1) |
| Pages | 63-70 |
| Published | 2000-9-1 |
| DOI | 10.1254/jjp.84.63 |
| PMID | 11043455 |
| MeSH | Acetylcysteine / pharmacology Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology Anti-Ulcer Agents / pharmacology* Apoptosis* Carnosine / analogs & derivatives* Carnosine / pharmacology* Cells, Cultured Cytochrome c Group / metabolism Dose-Response Relationship, Drug Drug Interactions Enzyme Activation / drug effects Gastric Mucosa / cytology Gastric Mucosa / drug effects* Indomethacin Organometallic Compounds / pharmacology* Peptide Hydrolases / metabolism Protective Agents / pharmacology Rats Rats, Wistar Reactive Oxygen Species / metabolism Time Factors Zinc / metabolism Zinc Compounds Zinc Sulfate / pharmacology |
| Times Cited | 27 |
| WOS Category | PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RGM1(RCB0876) |