| RRC ID |
39772
|
| Author |
Suizu F, Ueda K, Iwasaki T, Murata-Hori M, Hosoya H.
|
| Title |
Activation of actin-activated MgATPase activity of myosin II by phosphorylation with MAPK-activated protein kinase-1b (RSK-2).
|
| Journal |
J Biochem
|
| Abstract |
Regulatory light chain of myosin II (MRLC) was identified as a novel substrate of p90 ribosomal S6 kinase (RSK)-2, a Ser/Thr protein kinase which is phosphorylated and activated by mitogen-activated protein kinase (MAPK) in vitro and in vivo. Phosphopeptide map of MRLC phosphorylated by RSK-2 was identical to that by myosin light chain kinase (MLCK). Phosphoserine was recovered by the phosphoamino acid analysis of MRLC phosphorylated by RSK-2. Further, phosphorylation using recombinant glutathione S-transferase (GST) fusion proteins of HeLa MRLC2 revealed that RSK-2 phosphorylated wild-type MRLC2 (GST-wtMRLC2) but not its mutants GST-MRLC2(S19A) or GST-MRLC2(T18AS19A) (alanine substituted for Ser19 or both Ser19 and Thr18). These results revealed that RSK-2 phosphorylates MRLC at Ser19 as did MLCK. Phosphorylation of myosin II by RSK-2 resulted in activation of actin-activated MgATPase activity of myosin II. Interestingly, RSK-2 activity to phosphorylate MRLC was suppressed by phosphorylation with MAPK. RSK-2 might be a mediator that regulates myosin II activity through the MAPK cascade.
|
| Volume |
128(3)
|
| Pages |
435-40
|
| Published |
2000-9-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a022771
|
| PMID |
10965042
|
| MeSH |
Actins / metabolism*
Animals
Blotting, Western
Ca(2+) Mg(2+)-ATPase / metabolism*
Cells, Cultured
Chickens
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Gizzard, Avian
Glutathione Transferase / biosynthesis
Humans
MAP Kinase Kinase 1
Mitogen-Activated Protein Kinase Kinases / metabolism
Models, Molecular
Muscle, Smooth / enzymology
Myosins / metabolism*
Peptide Fragments / isolation & purification
Peptide Mapping
Phosphopeptides / isolation & purification
Phosphorylation
Plasmids
Precipitin Tests
Protein Serine-Threonine Kinases / metabolism
Recombinant Fusion Proteins / metabolism
Ribosomal Protein S6 Kinases / metabolism*
|
| IF |
2.476
|
| Times Cited |
23
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
